Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2027176..2027752 Replicon chromosome
Accession NZ_CP011456
Organism Anabaena sp. WA102 isolate

Toxin (Protein)


Gene name mazF Uniprot ID A0A0K2LXU4
Locus tag AA650_RS08965 Protein ID WP_028082683.1
Coordinates 2027393..2027752 (+) Length 120 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID K7W752
Locus tag AA650_RS08960 Protein ID WP_015083417.1
Coordinates 2027176..2027406 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AA650_RS08940 2023164..2023982 + 819 WP_053538743.1 WecB/TagA/CpsF family glycosyltransferase -
AA650_RS27740 2024783..2024932 + 150 WP_168636355.1 hypothetical protein -
AA650_RS08945 2024866..2025081 + 216 WP_053538744.1 type II toxin-antitoxin system HicA family toxin -
AA650_RS26290 2025122..2025214 + 93 Protein_1789 XisI protein -
AA650_RS27745 2025376..2025525 + 150 WP_168636354.1 hypothetical protein -
AA650_RS08950 2025601..2026296 + 696 Protein_1791 HNH endonuclease -
AA650_RS08955 2026438..2026755 + 318 WP_081424189.1 DUF3800 domain-containing protein -
AA650_RS08960 2027176..2027406 + 231 WP_015083417.1 hypothetical protein Antitoxin
AA650_RS08965 2027393..2027752 + 360 WP_028082683.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AA650_RS08970 2027885..2028085 + 201 WP_006278807.1 type II toxin-antitoxin system HicB family antitoxin -
AA650_RS08975 2028082..2028291 + 210 WP_053538746.1 type II toxin-antitoxin system HicA family toxin -
AA650_RS08980 2028609..2028911 + 303 WP_053538747.1 hypothetical protein -
AA650_RS08985 2028924..2029325 + 402 WP_053538748.1 type II toxin-antitoxin system VapC family toxin -
AA650_RS08990 2029409..2029684 - 276 WP_028082686.1 type II toxin-antitoxin system RelE/ParE family toxin -
AA650_RS08995 2029677..2029928 - 252 WP_053538749.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
AA650_RS09000 2030370..2030699 + 330 WP_053538750.1 hypothetical protein -
AA650_RS09005 2030706..2031155 + 450 WP_053538751.1 hypothetical protein -
AA650_RS09010 2031395..2031685 + 291 WP_053538752.1 hypothetical protein -
AA650_RS09015 2031682..2032026 + 345 WP_053538753.1 PIN domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-114)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13382.56 Da        Isoelectric Point: 6.3225

>T54276 WP_028082683.1 NZ_CP011456:2027393-2027752 [Anabaena sp. WA102]
MPEGNLTYKRGEIRWVNLDPTVGAEAQKIRACLIVQSDIMNQYGLLTIVMPFRPGSKQAPYIVNIKATVTNGLDKDRFID
VAQIRSVDYRRVLGLVGILEAEYWEEIRTALDVVLGFGV

Download         Length: 360 bp

>T54276 NZ_CP011456:2027393-2027752 [Anabaena sp. WA102]
ATGCCAGAGGGTAATTTAACTTATAAACGGGGTGAAATTCGCTGGGTAAATCTTGATCCAACTGTGGGAGCAGAAGCACA
AAAAATCCGTGCTTGTTTAATAGTTCAAAGTGATATTATGAATCAATATGGATTATTAACTATTGTGATGCCATTCCGAC
CAGGAAGTAAGCAAGCTCCCTATATTGTCAATATCAAAGCAACAGTAACTAATGGATTAGATAAAGATCGTTTTATTGAT
GTTGCTCAGATTCGTTCTGTAGATTATCGTCGGGTTTTAGGTTTGGTGGGAATTTTAGAAGCTGAATATTGGGAAGAAAT
TCGTACTGCTTTGGATGTGGTGTTAGGGTTTGGGGTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8563.66 Da        Isoelectric Point: 4.2958

>AT54276 WP_015083417.1 NZ_CP011456:2027176-2027406 [Anabaena sp. WA102]
MMKVTITLEEDILRFIDQQAKGNRSAYINALLAEQRRKILEAEIIAALQEDAKDLEYQNEISAWDNVAGDGINARG

Download         Length: 231 bp

>AT54276 NZ_CP011456:2027176-2027406 [Anabaena sp. WA102]
ATGATGAAAGTCACAATTACTTTGGAAGAAGACATTCTCAGATTTATTGATCAACAAGCAAAAGGTAATCGTAGTGCCTA
TATTAACGCACTATTAGCAGAACAAAGACGCAAAATTTTGGAAGCAGAAATAATTGCAGCACTCCAAGAAGATGCGAAAG
ATTTAGAATATCAAAATGAGATTTCTGCTTGGGATAATGTAGCTGGAGATGGTATTAATGCCAGAGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K2LXU4

Antitoxin

Source ID Structure
AlphaFold DB A0A0K2LY81

References