Detailed information of TA system TA054273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 450818..451337
• Replicon: chromosome
• Accession: NZ_CP011454
• Organism: Gemmatimonas phototrophica strain AP64

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GEMMAAP_RS01980
• Protein ID: WP_202969181.1
• Coordinates: 450818..451129 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A143BFY7
• Locus tag: GEMMAAP_RS01985
• Protein ID: WP_026849217.1
• Coordinates: 451110..451337 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEMMAAP_RS01970	446300..448108	-	1809	WP_026849215.1	PTS glucose transporter subunit IIBC	-
GEMMAAP_RS01975	448161..450719	-	2559	WP_075071598.1	phosphoenolpyruvate--protein phosphotransferase	-
GEMMAAP_RS01980	450818..451129	+	312	WP_202969181.1	BrnT family toxin	Toxin
GEMMAAP_RS01985	451110..451337	+	228	WP_026849217.1	hypothetical protein	Antitoxin
GEMMAAP_RS01990	451357..453588	+	2232	WP_053333946.1	protein kinase	-
GEMMAAP_RS20460	453612..453767	+	156	WP_158514691.1	hypothetical protein	-
GEMMAAP_RS01995	453771..454325	+	555	Protein_397	hypothetical protein	-
GEMMAAP_RS02000	454335..454745	-	411	WP_026849218.1	PIN domain-containing protein	-
GEMMAAP_RS02005	454745..454975	-	231	WP_145978954.1	ribbon-helix-helix domain-containing protein	-
GEMMAAP_RS02010	455161..455667	+	507	WP_053333948.1	CIA30 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (19-94)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11863.42 Da
• Isoelectric Point: 6.7200

>T54273 WP_202969181.1 NZ_CP011454:450818-451129 [Gemmatimonas phototrophica]
MCKRYTFYTKEGGFVEFEFDPLKSASNFAKHGIDFDAAQALWRDDRLLEVQARTTDEPRWLVVGRIAGVAWSAVVTYRDH
RVRLISVRHARAEEVALYEGEGL

Nucleotide

• Length: 312 bp

>T54273 NZ_CP011454:450818-451129 [Gemmatimonas phototrophica]
ATGTGCAAAAGGTATACTTTTTATACCAAAGAAGGGGGTTTTGTGGAGTTTGAGTTCGACCCGCTCAAGAGCGCCAGCAA
CTTCGCCAAACACGGCATCGACTTCGACGCCGCGCAGGCGCTGTGGCGTGACGACCGACTGCTCGAAGTGCAGGCGAGGA
CGACCGATGAACCACGGTGGCTCGTCGTGGGACGTATCGCGGGCGTCGCCTGGTCGGCGGTGGTCACGTATCGTGATCAC
CGTGTTCGTCTGATATCCGTGCGCCACGCGCGCGCTGAGGAGGTGGCGTTGTATGAAGGCGAAGGACTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8788.02 Da
• Isoelectric Point: 8.7839

>AT54273 WP_026849217.1 NZ_CP011454:451110-451337 [Gemmatimonas phototrophica]
MKAKDFDRKFDREVDITDDLDLSTARRPTQAPRRVNVDFPVWMIDSLDQEAARLGVTRQSIIKVWIAERLEKRAS

Nucleotide

• Length: 228 bp

>AT54273 NZ_CP011454:451110-451337 [Gemmatimonas phototrophica]
ATGAAGGCGAAGGACTTTGACCGCAAGTTCGACCGCGAGGTCGACATCACGGACGATCTCGATCTGTCCACTGCGCGTCG
CCCGACGCAAGCACCGCGTCGCGTGAACGTCGACTTTCCCGTCTGGATGATTGACTCGCTCGACCAGGAAGCTGCGAGAC
TCGGCGTCACGCGGCAGTCGATCATCAAGGTATGGATCGCCGAACGTCTCGAGAAGCGGGCGTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A143BFY7