Detailed information of TA system TA054241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1610847..1611455
• Replicon: chromosome
• Accession: NZ_CP011420
• Organism: Paenibacillus polymyxa strain ATCC 15970

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: VK72_RS06815
• Protein ID: WP_007429335.1
• Coordinates: 1611105..1611455 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: VK72_RS06810
• Protein ID: WP_170970806.1
• Coordinates: 1610847..1611098 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VK72_RS06795	1607048..1607680	+	633	WP_053324896.1	LysE family transporter	-
VK72_RS06800	1607791..1609068	+	1278	WP_023987594.1	outer membrane lipoprotein carrier protein LolA	-
VK72_RS06805	1609327..1610523	+	1197	WP_028543050.1	alanine racemase	-
VK72_RS06810	1610847..1611098	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
VK72_RS06815	1611105..1611455	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
VK72_RS06820	1611635..1613857	+	2223	WP_193217073.1	RNA-binding transcriptional accessory protein	-
VK72_RS27420	1613962..1614096	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
VK72_RS06825	1614241..1614765	+	525	WP_053324897.1	SprT family protein	-
VK72_RS06840	1615154..1616029	-	876	WP_028543047.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T54241 WP_007429335.1 NZ_CP011420:1611105-1611455 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T54241 NZ_CP011420:1611105-1611455 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCTATCACCGCTCAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTAGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT54241 WP_170970806.1 NZ_CP011420:1610847-1611098 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT54241 NZ_CP011420:1610847-1611098 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATCAACTTAACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1