Detailed information of TA system TA054226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 104447..105072
• Replicon: plasmid pCFSAN029787_01
• Accession: NZ_CP011417
• Organism: Escherichia coli strain CFSAN029787

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AA102_RS25645
• Protein ID: WP_046881633.1
• Coordinates: 104447..104845 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: AA102_RS25650
• Protein ID: WP_000450530.1
• Coordinates: 104845..105072 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA102_RS25625	99994..100503	+	510	WP_000628112.1	conjugal transfer entry exclusion protein TraS	-
AA102_RS25630	100517..101248	+	732	WP_000850424.1	complement resistance protein TraT	-
AA102_RS25635	101452..101931	+	480	WP_000282333.1	hypothetical protein	-
AA102_RS25640	102213..104438	+	2226	WP_046881631.1	type IV conjugative transfer system coupling protein TraD	-
AA102_RS25645	104447..104845	-	399	WP_046881633.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AA102_RS25650	104845..105072	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AA102_RS25660	105154..106688	+	1535	Protein_130	conjugative relaxase	-
AA102_RS25665	106781..107953	+	1173	WP_000952382.1	IS21-like element IS21 family transposase	-
AA102_RS25670	107953..108243	+	291	Protein_132	ATP-binding protein	-
AA102_RS25675	108419..109606	-	1188	WP_038341448.1	IS91 family transposase	-
AA102_RS25680	109606..109971	-	366	WP_000124126.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE1 / ospE2 / senB / faeC / faeD / faeE / faeF / faeH / faeI / ospC1 / ospD3/senA / ospD3/senA / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE	1..293826	293826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T54226 WP_046881633.1 NZ_CP011417:c104845-104447 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T54226 NZ_CP011417:c104845-104447 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT54226 WP_000450530.1 NZ_CP011417:c105072-104845 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT54226 NZ_CP011417:c105072-104845 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2