Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 46522..47047 | Replicon | plasmid pCFSAN029787_01 |
| Accession | NZ_CP011417 | ||
| Organism | Escherichia coli strain CFSAN029787 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | Q326Z8 |
| Locus tag | AA102_RS25290 | Protein ID | WP_001159860.1 |
| Coordinates | 46742..47047 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | Q7BEK0 |
| Locus tag | AA102_RS25285 | Protein ID | WP_000813626.1 |
| Coordinates | 46522..46740 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AA102_RS25240 | 41750..42034 | + | 285 | WP_038341659.1 | ribbon-helix-helix domain-containing protein | - |
| AA102_RS25245 | 42034..42309 | + | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| AA102_RS31885 | 42441..42578 | - | 138 | WP_000766699.1 | hypothetical protein | - |
| AA102_RS28960 | 42685..43036 | - | 352 | Protein_52 | plasmid partitioning/stability family protein | - |
| AA102_RS25255 | 43039..43607 | - | 569 | Protein_53 | plasmid segregation protein ParM | - |
| AA102_RS30185 | 43615..44706 | + | 1092 | Protein_54 | IS66 family transposase | - |
| AA102_RS28970 | 44901..46109 | - | 1209 | WP_000198523.1 | IS91 family transposase | - |
| AA102_RS25280 | 46090..46362 | - | 273 | WP_000019158.1 | hypothetical protein | - |
| AA102_RS25285 | 46522..46740 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| AA102_RS25290 | 46742..47047 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| AA102_RS30840 | 47083..47178 | + | 96 | Protein_59 | transposase domain-containing protein | - |
| AA102_RS25295 | 47440..49077 | + | 1638 | WP_046881589.1 | T3SS effector E3 ubiquitin-protein ligase IpaH9.8 | - |
| AA102_RS25300 | 49285..49554 | + | 270 | WP_000079956.1 | hypothetical protein | - |
| AA102_RS31890 | 49551..49777 | + | 227 | Protein_62 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| AA102_RS25305 | 49806..50291 | + | 486 | WP_038339729.1 | protein kinase | - |
| AA102_RS25310 | 50306..51003 | - | 698 | Protein_64 | IS1 family transposase | - |
| AA102_RS28985 | 51053..51172 | + | 120 | WP_071845702.1 | type III effector | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE1 / ospE2 / senB / faeC / faeD / faeE / faeF / faeH / faeI / ospC1 / ospD3/senA / ospD3/senA / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE | 1..293826 | 293826 | |
| - | inside | IScluster/Tn | - | ospI / ipaH9.8 / ospG | 28333..52660 | 24327 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11736.59 Da Isoelectric Point: 6.4674
>T54225 WP_001159860.1 NZ_CP011417:46742-47047 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T54225 NZ_CP011417:46742-47047 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCTTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCTTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8333.36 Da Isoelectric Point: 6.2838
>AT54225 WP_000813626.1 NZ_CP011417:46522-46740 [Escherichia coli]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
Download Length: 219 bp
>AT54225 NZ_CP011417:46522-46740 [Escherichia coli]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TTN3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q7BEK0 |