Detailed information of TA system TA054214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4116517..4116996
• Replicon: chromosome
• Accession: NZ_CP011416
• Organism: Escherichia coli strain CFSAN029787

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: AA102_RS20620
• Protein ID: WP_000938157.1
• Coordinates: 4116517..4116804 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: AA102_RS20625
• Protein ID: WP_001300743.1
• Coordinates: 4116805..4116996 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA102_RS20585	4113307..4113498	-	192	WP_001098752.1	DUF1482 family protein	-
AA102_RS20590	4113495..4113683	-	189	WP_000449193.1	cell division inhibition protein DicB	-
AA102_RS20595	4113694..4114542	-	849	WP_046881151.1	Rha family phage regulatory protein	-
AA102_RS31785	4115122..4115397	+	276	WP_000102679.1	hypothetical protein	-
AA102_RS20605	4115366..4115695	-	330	WP_046881152.1	hypothetical protein	-
AA102_RS20610	4115718..4115936	-	219	WP_046881153.1	hypothetical protein	-
AA102_RS20615	4116096..4116251	-	156	WP_000379557.1	DUF1391 family protein	-
AA102_RS20620	4116517..4116804	-	288	WP_000938157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AA102_RS20625	4116805..4116996	-	192	WP_001300743.1	hypothetical protein	Antitoxin
AA102_RS20630	4116965..4117426	-	462	WP_000538414.1	helix-turn-helix domain-containing protein	-
AA102_RS30705	4117457..4117828	-	372	WP_000448217.1	hypothetical protein	-
AA102_RS20645	4117931..4118212	+	282	WP_042188692.1	helix-turn-helix domain-containing protein	-
AA102_RS20650	4118216..4118641	+	426	WP_046881154.1	Rha family transcriptional regulator	-
AA102_RS30710	4118848..4119306	-	459	WP_073801390.1	hypothetical protein	-
AA102_RS20660	4119386..4120495	+	1110	WP_046881155.1	DNA-binding protein	-
AA102_RS20665	4120502..4121248	+	747	WP_046881156.1	ATP-binding protein	-
AA102_RS20670	4121263..4121685	+	423	WP_046881157.1	DUF977 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	4102185..4177738	75553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10967.74 Da
• Isoelectric Point: 6.7144

>T54214 WP_000938157.1 NZ_CP011416:c4116804-4116517 [Escherichia coli]
MLPVLWLESADADLDNITSYIARFDIDAAERLWKRLRTCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIE
IVNVVHARRQFPVTP

Nucleotide

• Length: 288 bp

>T54214 NZ_CP011416:c4116804-4116517 [Escherichia coli]
ATGTTACCGGTTTTATGGCTCGAAAGCGCAGATGCCGACCTAGATAATATAACCAGCTATATTGCACGTTTTGACATAGA
TGCGGCAGAACGTTTGTGGAAACGCTTAAGGACTTGCGTCCTGCCATTATCTGAACATCCGTATTTATACCCGCCAAGCG
ACAGAGTGCCTGGTTTGCGTGAAATTGTAGCTCACCCCAATTACATCATTCTGTACCGCGTGACAATTTCAGGCATTGAA
ATTGTTAACGTAGTTCACGCCCGCCGACAGTTCCCTGTAACACCTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7350.26 Da
• Isoelectric Point: 5.8256

>AT54214 WP_001300743.1 NZ_CP011416:c4116996-4116805 [Escherichia coli]
MNTALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFANMRKERSKQ

Nucleotide

• Length: 192 bp

>AT54214 NZ_CP011416:c4116996-4116805 [Escherichia coli]
ATGAACACGGCCCTTTCACCAATGGTTTCTGAATTTGAAACCGTCGAGCAAGAAAATAGTTATAACGAATGGCTGCGTGC
AAAAGTGGCCGCAAGCCTTGCTGATCCACGTCCAGCCATTCCCCATGACGAAGTTGAACGCAGAATGGCTGAACGCTTTG
CTAACATGCGCAAGGAACGGAGCAAACAGTAA