Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 3431602..3432178 Replicon chromosome
Accession NZ_CP011410
Organism Leptospira interrogans serovar Bratislava strain PigK151

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag BRAT_RS14555 Protein ID WP_000617906.1
Coordinates 3431602..3431943 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag BRAT_RS14560 Protein ID WP_000844758.1
Coordinates 3431930..3432178 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BRAT_RS14535 3427024..3427770 - 747 WP_000617026.1 GNAT family N-acetyltransferase -
BRAT_RS14540 3427888..3428775 - 888 WP_001071208.1 hypothetical protein -
BRAT_RS14545 3428847..3429440 - 594 WP_001226990.1 dihydrofolate reductase family protein -
BRAT_RS14550 3430118..3430630 - 513 WP_000079221.1 VOC family protein -
BRAT_RS14555 3431602..3431943 - 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BRAT_RS14560 3431930..3432178 - 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
BRAT_RS14575 3434232..3434528 - 297 WP_000477061.1 helix-turn-helix domain-containing protein -
BRAT_RS22985 3434682..3435140 + 459 WP_000453264.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T54200 WP_000617906.1 NZ_CP011410:c3431943-3431602 [Leptospira interrogans serovar Bratislava]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T54200 NZ_CP011410:c3431943-3431602 [Leptospira interrogans serovar Bratislava]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTTTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT54200 WP_000844758.1 NZ_CP011410:c3432178-3431930 [Leptospira interrogans serovar Bratislava]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT54200 NZ_CP011410:c3432178-3431930 [Leptospira interrogans serovar Bratislava]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References