Detailed information of TA system TA054199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3215840..3216468
• Replicon: chromosome
• Accession: NZ_CP011410
• Organism: Leptospira interrogans serovar Bratislava strain PigK151

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0F6IK20
• Locus tag: BRAT_RS13695
• Protein ID: WP_000281043.1
• Coordinates: 3216070..3216468 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8F7E3
• Locus tag: BRAT_RS13690
• Protein ID: WP_001192482.1
• Coordinates: 3215840..3216070 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRAT_RS22955	3210850..3211158	+	309	WP_002137686.1	hypothetical protein	-
BRAT_RS13660	3211486..3211695	-	210	WP_000041838.1	hypothetical protein	-
BRAT_RS13665	3211827..3212456	+	630	WP_001081629.1	helix-turn-helix domain-containing protein	-
BRAT_RS13670	3212651..3213574	+	924	WP_000680711.1	M28 family peptidase	-
BRAT_RS13675	3213800..3214489	+	690	WP_000590459.1	SH3 domain-containing protein	-
BRAT_RS13680	3214692..3215012	+	321	Protein_2604	hypothetical protein	-
BRAT_RS13685	3215019..3215549	+	531	WP_025181049.1	hypothetical protein	-
BRAT_RS13690	3215840..3216070	+	231	WP_001192482.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BRAT_RS13695	3216070..3216468	+	399	WP_000281043.1	type II toxin-antitoxin system VapC family toxin	Toxin
BRAT_RS13700	3216565..3217113	+	549	WP_000479566.1	hypothetical protein	-
BRAT_RS13705	3217256..3218332	+	1077	WP_001170563.1	hypothetical protein	-
BRAT_RS13710	3218333..3218791	+	459	WP_001239002.1	GNAT family N-acetyltransferase	-
BRAT_RS13715	3219008..3219868	-	861	WP_000682874.1	hypothetical protein	-
BRAT_RS13720	3220409..3221176	-	768	WP_000636954.1	DUF1554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15167.81 Da
• Isoelectric Point: 9.6893

>T54199 WP_000281043.1 NZ_CP011410:3216070-3216468 [Leptospira interrogans serovar Bratislava]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDVESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T54199 NZ_CP011410:3216070-3216468 [Leptospira interrogans serovar Bratislava]
ATGTATCTTTTGGATACCAATATTTGTATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGTAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8771.05 Da
• Isoelectric Point: 8.6588

>AT54199 WP_001192482.1 NZ_CP011410:3215840-3216070 [Leptospira interrogans serovar Bratislava]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT54199 NZ_CP011410:3215840-3216070 [Leptospira interrogans serovar Bratislava]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAAGCTGTAATATTAGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAACCGAAATCTGATAAAAGAGAAAAACTTTAA

Structures

Toxin

• AlphaFold DB: A0A0F6IK20

Antitoxin

• AlphaFold DB: A0A0F6HAF7