Detailed information of TA system TA054189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 13694..14271
• Replicon: plasmid pCFSAN008100
• Accession: NZ_CP011399
• Organism: Listeria monocytogenes strain CFSAN008100

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: CY94_RS15645
• Protein ID: WP_003728465.1
• Coordinates: 13694..14035 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: CY94_RS15650
• Protein ID: WP_003728464.1
• Coordinates: 14029..14271 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY94_RS15630	10173..10727	-	555	WP_003728468.1	recombinase family protein	-
CY94_RS15635	11007..11366	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
CY94_RS15640	11366..13501	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
CY94_RS15645	13694..14035	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CY94_RS15650	14029..14271	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CY94_RS15655	14332..14583	-	252	WP_003728463.1	hypothetical protein	-
CY94_RS15660	14714..15040	+	327	WP_003728462.1	hypothetical protein	-
CY94_RS15665	15040..15702	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
CY94_RS15670	15721..16098	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
CY94_RS15675	16257..16745	+	489	WP_003728520.1	hypothetical protein	-
CY94_RS15680	16867..17115	+	249	WP_003728519.1	hypothetical protein	-
CY94_RS15685	17116..17541	+	426	WP_003728518.1	hypothetical protein	-
CY94_RS15690	17545..18267	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
CY94_RS15695	18283..18999	+	717	WP_003731678.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68224	68224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T54189 WP_003728465.1 NZ_CP011399:c14035-13694 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T54189 NZ_CP011399:c14035-13694 [Listeria monocytogenes]
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Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT54189 WP_003728464.1 NZ_CP011399:c14271-14029 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT54189 NZ_CP011399:c14271-14029 [Listeria monocytogenes]
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TTT

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5