Detailed information of TA system TA054183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 1642873..1643667
• Replicon: chromosome
• Accession: NZ_CP011382
• Organism: Calothrix sp. 336/3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: IJ00_RS06555
• Protein ID: WP_201782696.1
• Coordinates: 1643185..1643667 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A0T7BPJ8
• Locus tag: IJ00_RS06550
• Protein ID: WP_035151183.1
• Coordinates: 1642873..1643160 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IJ00_RS06535	1638471..1640624	+	2154	WP_035151177.1	polysaccharide biosynthesis tyrosine autokinase	-
IJ00_RS06540	1640771..1642252	+	1482	WP_046814973.1	O-antigen ligase family protein	-
IJ00_RS06545	1642287..1642808	+	522	WP_035151181.1	serine acetyltransferase	-
IJ00_RS06550	1642873..1643160	+	288	WP_035151183.1	DUF1778 domain-containing protein	Antitoxin
IJ00_RS06555	1643185..1643667	+	483	WP_201782696.1	GNAT family N-acetyltransferase	Toxin
IJ00_RS06560	1643859..1644818	+	960	WP_035151195.1	glycosyltransferase	-
IJ00_RS06565	1645096..1646073	+	978	WP_035151196.1	glycosyltransferase	-
IJ00_RS06570	1646191..1647150	+	960	WP_035151197.1	glycosyltransferase family 2 protein	-
IJ00_RS26995	1647694..1648524	+	831	WP_052754404.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (65-141)

Antitoxin

• Vibrio phage ICP1, Orf50 (17-88)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17656.46 Da
• Isoelectric Point: 9.4472

>T54183 WP_201782696.1 NZ_CP011382:1643185-1643667 [Calothrix sp. 336/3]
IQAPQPLNQNHDWQEFDSGNEELDLWLKQRAWKNEGSGASRTYVVTVLEKVIAYYCLANGSVVNTTAPGKVRRNMPDPIP
VMVIGRLAVDRRWQGQGIGYALVRDGILRTLQAAKIAGIRAIIVHAISPQAKEFYEKCGFIASPVAPMTLMLPLTNIISG

Nucleotide

• Length: 483 bp

>T54183 NZ_CP011382:1643185-1643667 [Calothrix sp. 336/3]
ATTCAGGCTCCCCAACCTTTGAACCAAAACCATGATTGGCAAGAGTTTGACTCTGGTAACGAGGAATTAGATCTATGGTT
AAAGCAACGAGCATGGAAAAATGAGGGTAGTGGCGCATCTAGAACCTATGTAGTGACGGTGCTAGAAAAAGTCATTGCCT
ATTATTGTTTGGCAAATGGCAGTGTGGTTAATACTACTGCCCCAGGTAAAGTTAGACGCAATATGCCAGATCCCATTCCA
GTGATGGTGATTGGGCGATTAGCTGTAGATAGGCGTTGGCAGGGGCAAGGAATCGGCTATGCTTTGGTACGGGATGGGAT
TTTGCGTACTCTGCAAGCGGCAAAGATTGCGGGTATCAGGGCAATCATAGTTCACGCGATTTCCCCCCAGGCAAAGGAAT
TTTATGAGAAATGTGGCTTTATTGCTTCTCCCGTAGCACCAATGACGCTGATGCTGCCCCTCACTAATATCATATCTGGC
TGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10856.38 Da
• Isoelectric Point: 6.3053

>AT54183 WP_035151183.1 NZ_CP011382:1642873-1643160 [Calothrix sp. 336/3]
MQPNTAASKQPNRDFTINIRVQQGQRDLIDRAAAAVGKSRSDFILETACREAETVLLDRRLFLLDEQKFQEFIALLDTTP
NPQLQKLLTTKAPWE

Nucleotide

• Length: 288 bp

>AT54183 NZ_CP011382:1642873-1643160 [Calothrix sp. 336/3]
ATGCAGCCAAATACAGCAGCATCTAAACAGCCCAACCGCGACTTCACTATCAACATCAGGGTACAGCAAGGGCAACGAGA
TTTAATAGATCGAGCAGCAGCAGCAGTAGGTAAAAGTCGTTCTGATTTTATTTTAGAGACTGCTTGCCGAGAAGCGGAAA
CTGTTTTATTGGATCGTCGGCTTTTTTTGTTGGATGAACAAAAATTTCAGGAATTTATTGCCCTGCTCGACACCACACCA
AATCCCCAACTCCAGAAACTACTCACCACCAAAGCTCCTTGGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0T7BPJ8