Detailed information of TA system TA054182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 447986..448569
• Replicon: chromosome
• Accession: NZ_CP011382
• Organism: Calothrix sp. 336/3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IJ00_RS01855
• Protein ID: WP_035149479.1
• Coordinates: 448222..448569 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0T7BM98
• Locus tag: IJ00_RS01850
• Protein ID: WP_035149476.1
• Coordinates: 447986..448228 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IJ00_RS01825	443399..444289	-	891	WP_035149463.1	FHA domain-containing protein	-
IJ00_RS01830	444461..445186	-	726	WP_035149466.1	6-phosphogluconolactonase	-
IJ00_RS01835	445313..446053	-	741	WP_035149468.1	ribonuclease PH	-
IJ00_RS01840	446147..446731	-	585	WP_035149472.1	nucleoside monophosphate kinase	-
IJ00_RS01845	447199..447735	+	537	WP_035149474.1	P-loop NTPase family protein	-
IJ00_RS01850	447986..448228	+	243	WP_035149476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IJ00_RS01855	448222..448569	+	348	WP_035149479.1	endoribonuclease MazF	Toxin
IJ00_RS01860	448738..449574	-	837	WP_035149481.1	M56 family metallopeptidase	-
IJ00_RS01865	449574..449993	-	420	WP_035149483.1	BlaI/MecI/CopY family transcriptional regulator	-
IJ00_RS01870	450221..450859	+	639	WP_201782658.1	ThiF family adenylyltransferase	-
IJ00_RS01875	450954..451190	+	237	WP_035158296.1	hypothetical protein	-
IJ00_RS01880	451269..453239	-	1971	WP_035149486.1	acetate--CoA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-111)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13125.24 Da
• Isoelectric Point: 5.7237

>T54182 WP_035149479.1 NZ_CP011382:448222-448569 [Calothrix sp. 336/3]
MVASTERYIPDRGHIVYLDFDATKGHEQRGTRPAFVISPRSYNAKSSLALFMPITKQQKGYPFEVPLPLELQIQGVILAD
QIKCLDWKVRDIQFVETVPESLIEEVQAKIEPLIL

Nucleotide

• Length: 348 bp

>T54182 NZ_CP011382:448222-448569 [Calothrix sp. 336/3]
TTGGTAGCTTCAACAGAGCGCTATATTCCAGACAGAGGACACATCGTTTATCTAGACTTTGATGCCACTAAAGGTCATGA
ACAACGGGGAACCCGACCTGCCTTTGTGATTTCACCTCGTAGTTACAATGCAAAAAGCTCCCTAGCATTGTTTATGCCTA
TTACTAAACAGCAAAAGGGGTATCCCTTTGAGGTTCCCTTGCCCCTTGAGCTACAAATTCAAGGGGTAATACTGGCTGAT
CAAATCAAGTGTCTAGACTGGAAAGTCCGTGACATTCAGTTTGTTGAAACTGTTCCAGAAAGTTTGATTGAGGAGGTACA
GGCAAAAATTGAGCCACTAATCTTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8772.08 Da
• Isoelectric Point: 5.0509

>AT54182 WP_035149476.1 NZ_CP011382:447986-448228 [Calothrix sp. 336/3]
MVATVAKWGNSLAVRIPQHVAKEIQLTEGAEVDLVVIDGNLVIKPRLRRRYSLEELVAAITPENLHSEVETGVAAGNEVW

Nucleotide

• Length: 243 bp

>AT54182 NZ_CP011382:447986-448228 [Calothrix sp. 336/3]
ATGGTTGCGACAGTTGCTAAGTGGGGGAATAGTTTAGCTGTTCGGATTCCCCAGCATGTAGCCAAAGAGATTCAGTTAAC
TGAAGGTGCTGAGGTCGATCTTGTGGTGATCGACGGTAATCTAGTGATCAAACCTAGGCTTCGTAGACGCTATTCCTTGG
AAGAGTTAGTTGCCGCCATCACACCAGAGAATTTACATTCAGAAGTTGAGACTGGAGTCGCTGCGGGGAATGAAGTTTGG
TAG

Structures

Antitoxin

• AlphaFold DB: A0A0T7BM98