Detailed information of TA system TA054166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2258270..2258806
• Replicon: chromosome
• Accession: NZ_CP011366
• Organism: Salinicoccus halodurans strain H3B36 isolate soil

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7HLC0
• Locus tag: AAT16_RS11540
• Protein ID: WP_046790943.1
• Coordinates: 2258270..2258641 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AAT16_RS14720
• Protein ID: WP_200795322.1
• Coordinates: 2258642..2258806 (-)
• Length: 55 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAT16_RS11515	2253332..2255470	-	2139	WP_046790939.1	RNA-binding transcriptional accessory protein	-
AAT16_RS11520	2255572..2256348	-	777	WP_046790940.1	RNA polymerase sigma factor SigB	-
AAT16_RS11525	2256323..2256799	-	477	WP_046790941.1	anti-sigma B factor RsbW	-
AAT16_RS11530	2256801..2257127	-	327	WP_046790942.1	anti-sigma factor antagonist	-
AAT16_RS11535	2257206..2258222	-	1017	WP_200795323.1	PP2C family protein-serine/threonine phosphatase	-
AAT16_RS11540	2258270..2258641	-	372	WP_046790943.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AAT16_RS14720	2258642..2258806	-	165	WP_200795322.1	hypothetical protein	Antitoxin
AAT16_RS11545	2258875..2260026	-	1152	WP_046790944.1	alanine racemase	-
AAT16_RS11550	2260038..2260388	-	351	WP_046790945.1	holo-ACP synthase	-
AAT16_RS11555	2260385..2260858	-	474	WP_046790946.1	PH domain-containing protein	-
AAT16_RS11560	2260812..2262347	-	1536	WP_046790947.1	PH domain-containing protein	-
AAT16_RS11565	2262340..2262804	-	465	WP_046790948.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13790.04 Da
• Isoelectric Point: 10.2148

>T54166 WP_046790943.1 NZ_CP011366:c2258641-2258270 [Salinicoccus halodurans]
MKRGEVYLADLSPVQGSEQGGKRPVVIIQNNIGNYHSPTVIVAAITGRINKAKIPTHVEISKDTYNLDKDSVILLEQIRT
VDKKRLKEKLTYLNDDKMKEVDKALKISLELMPAKSKKSESKS

Nucleotide

• Length: 372 bp

>T54166 NZ_CP011366:c2258641-2258270 [Salinicoccus halodurans]
ATGAAAAGAGGAGAAGTATATCTTGCGGATCTATCACCGGTGCAGGGCTCTGAACAAGGCGGTAAACGCCCGGTAGTGAT
TATACAGAACAACATCGGCAACTATCACAGCCCGACTGTGATCGTTGCAGCCATTACCGGCAGAATCAACAAAGCCAAGA
TTCCGACGCATGTGGAGATATCCAAAGATACATATAATCTTGACAAGGACTCAGTGATACTTCTTGAACAGATAAGAACA
GTAGATAAGAAAAGGCTTAAAGAGAAGCTTACATATTTGAATGATGATAAGATGAAGGAAGTGGACAAAGCTTTGAAAAT
CAGTTTAGAACTCATGCCTGCCAAGTCCAAGAAATCTGAATCGAAATCCTGA

Antitoxin

Protein

• Length: 55 a.a.
• Molecular weight: 6092.65 Da
• Isoelectric Point: 3.4592

>AT54166 WP_200795322.1 NZ_CP011366:c2258806-2258642 [Salinicoccus halodurans]
MSNATLFGSIELSLEHGYIEMSEINLSIACESEELECEAWQSNEDFLVYSKDGE

Nucleotide

• Length: 165 bp

>AT54166 NZ_CP011366:c2258806-2258642 [Salinicoccus halodurans]
ATGAGTAATGCAACATTATTTGGCAGTATTGAACTTTCTCTTGAACATGGATATATTGAAATGAGCGAGATTAACCTCTC
GATTGCCTGTGAGAGCGAAGAGCTGGAATGCGAAGCATGGCAGTCGAATGAGGATTTTCTTGTTTACAGTAAAGATGGTG
AATAG

Structures

Toxin

• AlphaFold DB: A0A0F7HLC0