Detailed information of TA system TA054153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1147995..1149715
• Replicon: chromosome
• Accession: NZ_CP011364
• Organism: Edwardsiella sp. EA181011

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A034T636
• Locus tag: QY76_RS05170
• Protein ID: WP_045427186.1
• Coordinates: 1147995..1148651 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: QY76_RS05175
• Protein ID: WP_045427189.1
• Coordinates: 1148648..1149715 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QY76_RS05150	1143304..1143660	+	357	Protein_1001	integrase	-
QY76_RS05155	1144012..1144251	+	240	WP_012849883.1	hypothetical protein	-
QY76_RS05160	1144290..1146720	+	2431	Protein_1003	type I restriction-modification system subunit M	-
QY76_RS17395	1146720..1147982	+	1263	WP_045427183.1	restriction endonuclease subunit S	-
QY76_RS05170	1147995..1148651	+	657	WP_045427186.1	DUF4433 domain-containing protein	Toxin
QY76_RS05175	1148648..1149715	+	1068	WP_045427189.1	macro domain-containing protein	Antitoxin
QY76_RS05180	1149879..1152974	+	3096	WP_045427192.1	HsdR family type I site-specific deoxyribonuclease	-
QY76_RS05185	1152974..1153690	+	717	WP_012849890.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25052.40 Da
• Isoelectric Point: 6.6893

>T54153 WP_045427186.1 NZ_CP011364:1147995-1148651 [Edwardsiella sp. EA181011]
MAYDYSASLNPEKALIWRIVHRDNIPWILDNGLHCGNSQVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSSLRSVAAQNAPFVFTDSHAYYNWANYYTRLEDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLDGMVCYNNNVKVQLEDWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T54153 NZ_CP011364:1147995-1148651 [Edwardsiella sp. EA181011]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGGAAAAAGCCCTGATTTGGCGCATTGTCCATCGGGATAATATCCCGTG
GATACTGGACAACGGCCTGCACTGCGGCAACAGTCAAGTGCAGGCGGCAAACTGGATCAATATCGGCAACCCGGAACTTA
TCGGCAAACGCGCCGGGCATCCGGTGCCTGTCGGTATGGGCGGGACGCTGCATGACTACGTGCCGTTTTACTTTACCCCC
TTTTCGCCGATGCTGATGAATATCCACAGCGGGCGTGGCGGCATTACGCAGCGCCCCAATGAGGAAATCGTCATTCTGGT
CAGCAGCCTGCGAAGCGTGGCGGCGCAAAACGCCCCCTTCGTGTTTACCGACAGCCATGCCTACTATAACTGGGCCAACT
ACTACACCCGCCTGGAGGACCTGAATCAGATCGACTGGTCTATCTTACAGGCGCGGGATTTCCGTCGCGATCCAGACGAT
CCGGCTAAGTTTGAACGCTATCAGGCCGAGGCGTTAATCTGGAAGCACTGCCCCATCACACTGCTGGATGGGATGGTTTG
TTACAATAACAACGTCAAGGTACAATTAGAAGACTGGCTGTCGCAACGTAATTTGACAATGCCCGTTCACGCACGGGCCG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40688.99 Da
• Isoelectric Point: 6.6116

>AT54153 WP_045427189.1 NZ_CP011364:1148648-1149715 [Edwardsiella sp. EA181011]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYADACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMVWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT54153 NZ_CP011364:1148648-1149715 [Edwardsiella sp. EA181011]
ATGATCACATATACACAAGGCAATTTACTGGACGCGCCTGTGGAAGCACTGGTGAATACGGTGAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTTAAAGAGCGCTTCCCCGACAATATGAAAGCCTATGCCGACGCCTGCAAACAAAAGC
AGGTGATGACTGGCAAGATGTTTATTACCGAAACTGGCGAGCTGATGGGGCCACGCTGGATCGTCAACTTCCCTACCAAG
CAGCACTGGCGCGCCGACTCCCGCATGGTGTGGATTGAAGACGGTTTGCAGGACTTGCGCCGCTTTTTGATCGAAAAGAA
AGTGCGGTCCATCGCCATTCCCCCGCTGGGCGCGGGCAACGGCGGCCTGAACTGGCCGGATGTCCGGGCGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATCCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGCAGC
GGTGTAAAAAAGCTCACCCCCGCCAGAGCGATGATCGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGTATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGCTACAACGCGCCATTGAACAGCACCAGCAGGAGGACGTGCTAAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTATGCTCCTAACCTGAACCATCTGCTTAACGCGCTGGACGGTACCTATCTGAAA
GCCGAGAAACGTATCCCGGACAGCCAGCCACTGGATGTGATCTGGTTTAACGATCGGGAAAAAGAACACATCAACCTATG
GTTGAACAGCGAAGCCAAAGCGTGGTTGCCAGCGCTTGAGCAGGTCAGCCAGTTGATTGACGGTTTTGAATCGCCGTTTG
GTCTGGAGCTGTTGGCGACCGTTGACTGGTTGTTGACCCGGGGACAATGCCAGCCGACGCTGGACTCGGTGAAAGAGGGG
TTGCATCAGTGGCCTGCGGGAGAACCTTGGGCCAGCCGTAAGATAAAGCTGTTTGACGATAAGAATCTGCAATTCGCCAT
TAACCGCGTGATGGAGTTTCATTGCTGA

Structures

Toxin

• AlphaFold DB: A0A034T636