Detailed information of TA system TA054129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3525162..3525799
• Replicon: chromosome
• Accession: NZ_CP011347
• Organism: Bacillus velezensis strain YJ11-1-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AAV30_RS16780
• Protein ID: WP_003156187.1
• Coordinates: 3525162..3525512 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AAV30_RS16785
• Protein ID: WP_003156188.1
• Coordinates: 3525518..3525799 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAV30_RS16740	3520202..3520804	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
AAV30_RS16745	3520804..3521592	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
AAV30_RS16750	3521558..3522040	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
AAV30_RS16755	3522037..3522366	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
AAV30_RS16760	3522430..3523437	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
AAV30_RS16765	3523449..3523850	-	402	WP_003156178.1	anti-sigma regulatory factor	-
AAV30_RS16770	3523853..3524218	-	366	WP_007609584.1	RsbT antagonist protein RsbS	-
AAV30_RS16775	3524223..3525044	-	822	WP_003156182.1	STAS domain-containing protein	-
AAV30_RS16780	3525162..3525512	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AAV30_RS16785	3525518..3525799	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AAV30_RS16790	3525919..3527088	-	1170	WP_038457028.1	alanine racemase	-
AAV30_RS16795	3527205..3528212	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
AAV30_RS16800	3528377..3528742	-	366	WP_007609580.1	holo-ACP synthase	-
AAV30_RS16805	3528835..3529434	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T54129 WP_003156187.1 NZ_CP011347:c3525512-3525162 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T54129 NZ_CP011347:c3525512-3525162 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT54129 WP_003156188.1 NZ_CP011347:c3525799-3525518 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT54129 NZ_CP011347:c3525799-3525518 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5