Detailed information of TA system TA054128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2733370..2734287
• Replicon: chromosome
• Accession: NZ_CP011347
• Organism: Bacillus velezensis strain YJ11-1-4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: AAV30_RS12985
• Protein ID: WP_007407256.1
• Coordinates: 2733370..2734116 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: AAV30_RS12990
• Protein ID: WP_003154807.1
• Coordinates: 2734117..2734287 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAV30_RS12965	2728763..2729713	-	951	WP_007610844.1	ring-cleaving dioxygenase	-
AAV30_RS12970	2730036..2731352	+	1317	WP_038457739.1	amino acid permease	-
AAV30_RS12975	2731637..2732254	+	618	WP_032874614.1	DUF47 domain-containing protein	-
AAV30_RS12980	2732267..2733265	+	999	WP_032874611.1	inorganic phosphate transporter	-
AAV30_RS12985	2733370..2734116	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
AAV30_RS12990	2734117..2734287	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
AAV30_RS12995	2734544..2735422	-	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
AAV30_RS13000	2735436..2735699	-	264	WP_003154813.1	phage holin	-
AAV30_RS13005	2735713..2735976	-	264	WP_003154815.1	hemolysin XhlA family protein	-
AAV30_RS13010	2736029..2736790	-	762	WP_038457737.1	hypothetical protein	-
AAV30_RS13015	2736847..2737044	-	198	WP_007610833.1	XkdX family protein	-
AAV30_RS13020	2737049..2737420	-	372	WP_038457736.1	YomQ/XkdW protein, phage-like element PBSX	-
AAV30_RS19705	2737433..2737900	-	468	WP_076851257.1	hypothetical protein	-
AAV30_RS13030	2738289..2738510	-	222	Protein_2616	terminase	-
AAV30_RS13035	2738623..2739135	-	513	WP_032874577.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T54128 WP_007407256.1 NZ_CP011347:2733370-2734116 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T54128 NZ_CP011347:2733370-2734116 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT54128 WP_003154807.1 NZ_CP011347:2734117-2734287 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT54128 NZ_CP011347:2734117-2734287 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTTAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14