Detailed information of TA system TA054125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 475566..476203
• Replicon: chromosome
• Accession: NZ_CP011346
• Organism: Bacillus velezensis strain JJ-D34

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AAV29_RS02410
• Protein ID: WP_003156187.1
• Coordinates: 475853..476203 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AAV29_RS02405
• Protein ID: WP_003156188.1
• Coordinates: 475566..475847 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAV29_RS02385	471931..472530	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
AAV29_RS02390	472623..472988	+	366	WP_014304402.1	holo-ACP synthase	-
AAV29_RS02395	473153..474160	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
AAV29_RS02400	474277..475446	+	1170	WP_003156189.1	alanine racemase	-
AAV29_RS02405	475566..475847	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AAV29_RS02410	475853..476203	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AAV29_RS02415	476321..477142	+	822	WP_003156182.1	STAS domain-containing protein	-
AAV29_RS02420	477147..477512	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
AAV29_RS02425	477515..477916	+	402	WP_003156178.1	anti-sigma regulatory factor	-
AAV29_RS02430	477928..478935	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
AAV29_RS02435	478999..479328	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
AAV29_RS02440	479325..479807	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
AAV29_RS02445	479773..480561	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
AAV29_RS02450	480561..481163	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T54125 WP_003156187.1 NZ_CP011346:475853-476203 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T54125 NZ_CP011346:475853-476203 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT54125 WP_003156188.1 NZ_CP011346:475566-475847 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT54125 NZ_CP011346:475566-475847 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5