Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3015488..3016142 Replicon chromosome
Accession NZ_CP011343
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag U069_RS14895 Protein ID WP_000244777.1
Coordinates 3015488..3015895 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag U069_RS14900 Protein ID WP_000354046.1
Coordinates 3015876..3016142 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U069_RS14875 3011445..3013178 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
U069_RS14880 3013184..3013894 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
U069_RS14885 3013919..3014815 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
U069_RS14890 3014927..3015448 + 522 WP_001055874.1 flavodoxin FldB -
U069_RS14895 3015488..3015895 - 408 WP_000244777.1 toxin CptA Toxin
U069_RS14900 3015876..3016142 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
U069_RS14905 3016385..3017365 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
U069_RS14910 3017561..3018220 - 660 WP_000250274.1 hemolysin III family protein -
U069_RS14915 3018384..3018695 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
U069_RS14920 3018740..3020173 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
U069_RS14925 3020230..3020973 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T54113 WP_000244777.1 NZ_CP011343:c3015895-3015488 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T54113 NZ_CP011343:c3015895-3015488 [Escherichia coli K-12]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT54113 WP_000354046.1 NZ_CP011343:c3016142-3015876 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT54113 NZ_CP011343:c3016142-3015876 [Escherichia coli K-12]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References