Detailed information of TA system TA054088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 478443..479061
• Replicon: chromosome
• Accession: NZ_CP011343
• Organism: Escherichia coli K-12 substr. GM4792

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: U069_RS02370
• Protein ID: WP_001290581.1
• Coordinates: 478443..478661 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: U069_RS02375
• Protein ID: WP_000344800.1
• Coordinates: 478687..479061 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U069_RS02340	473732..474304	+	573	WP_000779842.1	YbaY family lipoprotein	-
U069_RS02345	474335..474646	-	312	WP_000409911.1	MGMT family protein	-
U069_RS02350	475025..475378	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
U069_RS02355	475420..476970	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
U069_RS02360	477134..477604	-	471	WP_000136192.1	YlaC family protein	-
U069_RS02365	477720..478271	-	552	WP_000102564.1	maltose O-acetyltransferase	-
U069_RS02370	478443..478661	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
U069_RS02375	478687..479061	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
U069_RS02380	479607..482756	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
U069_RS02385	482779..483972	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T54088 WP_001290581.1 NZ_CP011343:c478661-478443 [Escherichia coli K-12]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T54088 NZ_CP011343:c478661-478443 [Escherichia coli K-12]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT54088 WP_000344800.1 NZ_CP011343:c479061-478687 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT54088 NZ_CP011343:c479061-478687 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5