Detailed information of TA system TA054087

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 262466..263145
• Replicon: chromosome
• Accession: NZ_CP011343
• Organism: Escherichia coli K-12 substr. GM4792

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: U069_RS01250
• Protein ID: WP_000854672.1
• Coordinates: 262466..262807 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: U069_RS01255
• Protein ID: WP_000070395.1
• Coordinates: 262828..263145 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U069_RS01225	257743..258144	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
U069_RS01230	258183..259238	-	1056	WP_000749863.1	phosphoporin PhoE	-
U069_RS01235	259526..260629	+	1104	WP_001285288.1	glutamate 5-kinase	-
U069_RS01240	260641..261894	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
U069_RS01250	262466..262807	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
U069_RS01255	262828..263145	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
U069_RS23025	263164..263385	-	222	WP_000691994.1	DUF987 domain-containing protein	-
U069_RS01260	263394..263870	-	477	WP_000811693.1	RadC family protein	-
U069_RS01265	263886..264344	-	459	WP_000211838.1	antirestriction protein	-
U069_RS01270	264442..264681	-	240	WP_000194654.1	DUF905 domain-containing protein	-
U069_RS01275	264758..265225	-	468	WP_001547765.1	hypothetical protein	-
U069_RS25225	265248..265691	-	444	WP_000824223.1	hypothetical protein	-
U069_RS25230	265691..265918	-	228	WP_001548158.1	hypothetical protein	-
U069_RS25235	265914..266105	-	192	Protein_249	DeoR family transcriptional regulator	-
U069_RS01290	266322..267143	-	822	WP_000197389.1	DUF945 domain-containing protein	-
U069_RS01295	267235..268098	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T54087 WP_000854672.1 NZ_CP011343:c262807-262466 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T54087 NZ_CP011343:c262807-262466 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT54087 WP_000070395.1 NZ_CP011343:c263145-262828 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT54087 NZ_CP011343:c263145-262828 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684