Detailed information of TA system TA054058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1491154..1491792
• Replicon: chromosome
• Accession: NZ_CP011342
• Organism: Escherichia coli K-12 substr. GM4792

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: U068_RS07455
• Protein ID: WP_000813794.1
• Coordinates: 1491154..1491330 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: U068_RS07460
• Protein ID: WP_001270286.1
• Coordinates: 1491376..1491792 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U068_RS07435	1486773..1487987	-	1215	WP_001326689.1	BenE family transporter YdcO	-
U068_RS07440	1488040..1488576	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
U068_RS07445	1488649..1490610	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
U068_RS07450	1490702..1490932	-	231	WP_000494244.1	YncJ family protein	-
U068_RS07455	1491154..1491330	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
U068_RS07460	1491376..1491792	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
U068_RS07465	1491871..1493277	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
U068_RS07470	1493522..1494667	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
U068_RS07475	1494685..1495698	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
U068_RS07480	1495699..1496640	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T54058 WP_000813794.1 NZ_CP011342:1491154-1491330 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T54058 NZ_CP011342:1491154-1491330 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT54058 WP_001270286.1 NZ_CP011342:1491376-1491792 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT54058 NZ_CP011342:1491376-1491792 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB