Detailed information of TA system TA054053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 478879..479497
• Replicon: chromosome
• Accession: NZ_CP011342
• Organism: Escherichia coli K-12 substr. GM4792

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: U068_RS02365
• Protein ID: WP_001290581.1
• Coordinates: 478879..479097 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: U068_RS02370
• Protein ID: WP_000344800.1
• Coordinates: 479123..479497 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U068_RS02330	474168..474740	+	573	WP_000779842.1	YbaY family lipoprotein	-
U068_RS02335	474771..475082	-	312	WP_000409911.1	MGMT family protein	-
U068_RS02345	475461..475814	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
U068_RS02350	475856..477406	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
U068_RS02355	477570..478040	-	471	WP_000136192.1	YlaC family protein	-
U068_RS02360	478156..478707	-	552	WP_000102564.1	maltose O-acetyltransferase	-
U068_RS02365	478879..479097	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
U068_RS02370	479123..479497	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
U068_RS02375	480043..483192	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
U068_RS02380	483215..484408	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T54053 WP_001290581.1 NZ_CP011342:c479097-478879 [Escherichia coli K-12]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T54053 NZ_CP011342:c479097-478879 [Escherichia coli K-12]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT54053 WP_000344800.1 NZ_CP011342:c479497-479123 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT54053 NZ_CP011342:c479497-479123 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5