Detailed information of TA system TA054046

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 4658549..4659408
• Replicon: chromosome
• Accession: NZ_CP011339
• Organism: Microcystis panniformis FACHB-1757

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: I4HG17
• Locus tag: VL20_RS22120
• Protein ID: WP_002791882.1
• Coordinates: 4659010..4659408 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: A0A1X9LBQ1
• Locus tag: VL20_RS22115
• Protein ID: WP_002742890.1
• Coordinates: 4658549..4658968 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VL20_RS22095 (VL20_5350)	4654276..4655139	-	864	WP_052277817.1	WYL domain-containing protein	-
VL20_RS22100 (VL20_5351)	4655239..4657404	+	2166	WP_052277818.1	type I-D CRISPR-associated helicase Cas3'	-
VL20_RS22105 (VL20_5352)	4657451..4657693	+	243	WP_052277819.1	hypothetical protein	-
VL20_RS22110 (VL20_5353)	4658039..4658386	+	348	WP_002742891.1	hypothetical protein	-
VL20_RS22115 (VL20_5354)	4658549..4658968	+	420	WP_002742890.1	nucleotidyltransferase domain-containing protein	Antitoxin
VL20_RS22120 (VL20_5355)	4659010..4659408	+	399	WP_002791882.1	DUF86 domain-containing protein	Toxin
VL20_RS22125 (VL20_5356)	4659401..4659757	+	357	WP_284525888.1	nucleotidyltransferase domain-containing protein	-
VL20_RS33180 (VL20_5357)	4659944..4660087	+	144	WP_002742887.1	hypothetical protein	-
VL20_RS22130 (VL20_5358)	4660080..4663523	+	3444	WP_052277820.1	type I-D CRISPR-associated protein Cas10d/Csc3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4653008..4654204	1196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (1-117)

Antitoxin

• Polymerase beta, nucleotidyltransferase (6-101)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15326.56 Da
• Isoelectric Point: 4.6345

>T54046 WP_002791882.1 NZ_CP011339:4659010-4659408 [Microcystis panniformis FACHB-1757]
MNQYLAELSEYGSITLEDYRTLRERQLAIERLIQLIVQTGIDINYQILKCLDIESPNNARDALFQIVELGILEEHLAVQL
AESIKLRNLLVHLYKKIDPDIVHSSIANILRDYPRYQRSIVQYLDSLEAENG

Nucleotide

• Length: 399 bp

>T54046 NZ_CP011339:4659010-4659408 [Microcystis panniformis FACHB-1757]
ATGAATCAATACCTCGCCGAACTTAGCGAGTACGGTTCGATCACCTTAGAGGATTATCGTACATTAAGAGAACGTCAATT
AGCCATAGAAAGGTTAATTCAGCTTATCGTTCAAACTGGCATCGATATCAATTACCAGATTTTAAAATGTTTAGATATCG
AGTCTCCGAATAATGCTCGCGACGCATTATTTCAAATCGTAGAACTGGGAATCTTAGAAGAACACCTAGCCGTTCAACTG
GCCGAGTCTATTAAATTAAGAAACTTACTCGTACACTTATACAAGAAAATTGATCCCGATATTGTTCATTCCTCGATCGC
TAATATTTTACGAGATTATCCTCGCTACCAACGCTCGATCGTTCAATATCTAGATTCCTTGGAGGCAGAAAATGGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16307.78 Da
• Isoelectric Point: 5.1063

>AT54046 WP_002742890.1 NZ_CP011339:4658549-4658968 [Microcystis panniformis FACHB-1757]
MNRLVEKITAIVEKLPNLKLLILFGSRARREHRPDSDWDFAVLYEERSDQKDISSLLKIYTLLEQALEIPEDKIDVVDLK
ECSPILAHYVARDGQLLYERETGLFEGFKEKFLMNPEESKALYRQLRNNLEISLREKGV

Nucleotide

• Length: 420 bp

>AT54046 NZ_CP011339:4658549-4658968 [Microcystis panniformis FACHB-1757]
ATGAATAGGCTAGTAGAAAAAATTACGGCTATTGTCGAAAAACTACCGAATTTAAAACTATTAATTCTCTTCGGTTCTAG
AGCGCGTAGAGAGCATAGACCTGATAGCGATTGGGATTTCGCTGTTTTATACGAGGAAAGAAGCGATCAAAAGGATATTT
CGAGTCTGTTGAAAATTTATACACTGCTCGAACAAGCTTTAGAGATTCCCGAGGACAAAATTGATGTGGTTGATTTAAAA
GAATGTTCGCCGATCCTCGCTCATTATGTCGCACGGGATGGTCAACTTCTATACGAACGGGAAACGGGACTATTCGAGGG
ATTTAAAGAAAAATTTCTCATGAATCCTGAAGAATCGAAAGCGTTATACCGTCAATTGCGGAATAATCTGGAGATTTCTC
TACGAGAAAAAGGAGTGTAA

Structures

Toxin

• AlphaFold DB: I4HG17

Antitoxin

• AlphaFold DB: A0A1X9LBQ1