Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/COG3657-dnstrm_HI1420
Location 2894661..2895251 Replicon chromosome
Accession NZ_CP011339
Organism Microcystis panniformis FACHB-1757

Toxin (Protein)


Gene name PumA Uniprot ID A0A0F6RKM7
Locus tag VL20_RS13900 Protein ID WP_046661678.1
Coordinates 2894661..2894951 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I4ID81
Locus tag VL20_RS13905 Protein ID WP_002757266.1
Coordinates 2894961..2895251 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VL20_RS13865 (VL20_3369) 2890015..2890443 + 429 WP_052276834.1 hypothetical protein -
VL20_RS13870 (VL20_3370) 2890977..2891474 - 498 WP_249265012.1 type II toxin-antitoxin system VapC family toxin -
VL20_RS13875 (VL20_3371) 2891471..2891716 - 246 WP_008201147.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VL20_RS13880 2891773..2892039 + 267 WP_052276835.1 DUF433 domain-containing protein -
VL20_RS31190 2892020..2892346 + 327 WP_052276836.1 DUF5615 family PIN-like protein -
VL20_RS31195 2892385..2892681 - 297 Protein_2900 arginase family protein -
VL20_RS32720 (VL20_3374) 2892668..2892796 - 129 WP_284525783.1 hypothetical protein -
VL20_RS13890 (VL20_3375) 2893031..2893258 + 228 WP_052276837.1 hypothetical protein -
VL20_RS28770 2893255..2893528 + 274 Protein_2903 hypothetical protein -
VL20_RS13895 (VL20_3377) 2893890..2894519 + 630 WP_128575219.1 hypothetical protein -
VL20_RS13900 (VL20_3378) 2894661..2894951 + 291 WP_046661678.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VL20_RS13905 (VL20_3379) 2894961..2895251 + 291 WP_002757266.1 putative addiction module antidote protein Antitoxin
VL20_RS13910 (VL20_3381) 2895521..2896321 + 801 WP_052276839.1 uroporphyrinogen-III C-methyltransferase -
VL20_RS13915 (VL20_3382) 2896332..2896751 + 420 WP_002757262.1 secondary thiamine-phosphate synthase enzyme YjbQ -
VL20_RS13920 (VL20_3383) 2896884..2897378 + 495 WP_002735968.1 photosystem I reaction center subunit III -
VL20_RS13925 (VL20_3384) 2897424..2897552 + 129 WP_002738148.1 photosystem I reaction center subunit IX -
VL20_RS13930 (VL20_3385) 2897719..2899317 + 1599 WP_052278471.1 peptide ligase PGM1-related protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Uncharacterized protein HI1420

(7-92)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11054.65 Da        Isoelectric Point: 10.1314

>T54043 WP_046661678.1 NZ_CP011339:2894661-2894951 [Microcystis panniformis FACHB-1757]
MIEIRQTETYSQWFSNLRDRQAKARIDIRVRRLSMGNPGDVKPVGKGVSELRIDYGQGYRVYFIQRGETLIILLAAGDKQ
TQERDIKTALNLAQDL

Download         Length: 291 bp

>T54043 NZ_CP011339:2894661-2894951 [Microcystis panniformis FACHB-1757]
ATGATTGAAATTCGCCAGACCGAAACCTACTCTCAGTGGTTTAGCAATTTGCGGGATCGCCAAGCAAAAGCACGTATAGA
CATTCGTGTTCGTCGCCTGTCTATGGGCAATCCAGGCGATGTTAAACCAGTAGGAAAAGGTGTATCGGAACTGCGGATCG
ATTATGGCCAAGGCTATCGGGTGTATTTCATTCAGCGAGGAGAGACCTTGATCATCCTCTTGGCAGCAGGAGATAAGCAA
ACTCAAGAACGGGACATCAAAACAGCATTAAATTTAGCACAAGACTTATAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10320.80 Da        Isoelectric Point: 4.4475

>AT54043 WP_002757266.1 NZ_CP011339:2894961-2895251 [Microcystis panniformis FACHB-1757]
MATIQTYPWDAAEHLETKEDIAAYLEAALEDGDPSLVVAALGDIARSKGMTHIARETGLGRESLYKALSIEGNPEFATVL
KVLQSLGLRLQVVPIA

Download         Length: 291 bp

>AT54043 NZ_CP011339:2894961-2895251 [Microcystis panniformis FACHB-1757]
ATGGCTACTATCCAAACCTATCCTTGGGATGCGGCAGAACATCTCGAAACAAAAGAAGATATAGCTGCATATCTTGAAGC
AGCCCTTGAAGATGGCGATCCAAGCTTAGTGGTAGCAGCATTAGGTGATATTGCTCGATCTAAAGGGATGACACATATTG
CCCGTGAAACAGGGTTGGGGCGTGAAAGTCTCTATAAAGCTTTGTCAATCGAAGGCAATCCAGAGTTTGCCACAGTTCTT
AAGGTCTTACAATCACTTGGGTTACGCCTCCAAGTCGTACCAATTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6RKM7

Antitoxin

Source ID Structure
AlphaFold DB I4ID81

References