Detailed information of TA system TA054039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT_toxin-BrnA
• Location: 2301850..2302342
• Replicon: chromosome
• Accession: NZ_CP011339
• Organism: Microcystis panniformis FACHB-1757

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: I4GAG4
• Locus tag: VL20_RS11040
• Protein ID: WP_002772034.1
• Coordinates: 2302064..2302342 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: VL20_RS11035
• Protein ID: WP_081417880.1
• Coordinates: 2301850..2302059 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VL20_RS11010 (VL20_2676)	2297287..2297565	+	279	WP_052276534.1	hemolysin XhlA family protein	-
VL20_RS11015 (VL20_2677)	2297942..2298217	-	276	WP_284526095.1	type II toxin-antitoxin system HicB family antitoxin	-
VL20_RS32560	2298320..2298469	-	150	WP_249264921.1	DUF86 domain-containing protein	-
VL20_RS32565 (VL20_2679)	2298651..2298893	-	243	WP_284526096.1	nucleotidyltransferase domain-containing protein	-
VL20_RS11030 (VL20_2681)	2299399..2301401	-	2003	Protein_2301	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
VL20_RS31120	2301502..2301663	-	162	WP_158499339.1	hypothetical protein	-
VL20_RS11035 (VL20_2682)	2301850..2302059	-	210	WP_081417880.1	BrnA antitoxin family protein	Antitoxin
VL20_RS11040	2302064..2302342	-	279	WP_002772034.1	BrnT family toxin	Toxin
VL20_RS11045 (VL20_2684)	2302456..2302791	-	336	WP_052276537.1	DUF86 domain-containing protein	-
VL20_RS11050 (VL20_2685)	2302788..2303081	-	294	WP_002770523.1	nucleotidyltransferase family protein	-
VL20_RS11055 (VL20_2686)	2303144..2303911	-	768	WP_052276538.1	PP2C family serine/threonine-protein phosphatase	-
VL20_RS11060 (VL20_2687)	2303925..2304581	-	657	WP_052276539.1	VWA domain-containing protein	-
VL20_RS11065 (VL20_2689)	2304817..2305158	-	342	WP_052278442.1	thioredoxin domain-containing protein	-
VL20_RS11070 (VL20_2690)	2305247..2305948	-	702	WP_128575193.1	NnrU family protein	-
VL20_RS11075 (VL20_2691)	2306275..2306574	+	300	WP_002786792.1	(2Fe-2S) ferredoxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2264131..2305948	41817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10835.42 Da
• Isoelectric Point: 9.9743

>T54039 WP_002772034.1 NZ_CP011339:c2302342-2302064 [Microcystis panniformis FACHB-1757]
MKYEWDQDKSDANLEKHGLSFEDAPLVFGGKCVTFEDSRFVYGEIRYITLGKLKGRVVLIVHTPRSNKTRIISMRKANNR
EQKIYQKRLEEN

Nucleotide

• Length: 279 bp

>T54039 NZ_CP011339:c2302342-2302064 [Microcystis panniformis FACHB-1757]
ATGAAGTACGAATGGGATCAAGACAAGAGCGACGCTAACTTAGAGAAGCACGGACTGAGTTTTGAGGATGCACCGCTAGT
TTTTGGTGGTAAATGTGTTACCTTCGAGGATAGCCGTTTCGTTTATGGTGAGATAAGATATATTACGCTTGGCAAATTGA
AAGGTCGAGTTGTCTTAATCGTCCATACCCCTCGCTCTAATAAAACTCGTATAATCTCAATGAGAAAGGCAAACAATCGT
GAGCAAAAAATCTATCAAAAGCGACTTGAAGAGAATTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8117.96 Da
• Isoelectric Point: 3.7178

>AT54039 WP_081417880.1 NZ_CP011339:c2302059-2301850 [Microcystis panniformis FACHB-1757]
MTDENIDYSDIPPLGDEFFTQETVSFSTSKQQLTIQLDQDILEWLKAQGKGYETQINYILREVMEQQQQ

Nucleotide

• Length: 210 bp

>AT54039 NZ_CP011339:c2302059-2301850 [Microcystis panniformis FACHB-1757]
ATGACGGATGAGAATATCGATTATTCTGATATTCCGCCATTAGGTGATGAATTCTTTACCCAAGAAACAGTATCTTTTTC
GACATCAAAGCAGCAATTAACCATCCAACTAGATCAAGATATATTAGAATGGTTAAAAGCTCAAGGGAAAGGATATGAGA
CACAAATTAACTACATCTTGCGGGAAGTGATGGAACAGCAGCAGCAGTAA

Structures

Toxin

• AlphaFold DB: I4GAG4