Detailed information of TA system TA054038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1464242..1464855
• Replicon: chromosome
• Accession: NZ_CP011339
• Organism: Microcystis panniformis FACHB-1757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I4HQS4
• Locus tag: VL20_RS07160
• Protein ID: WP_002793526.1
• Coordinates: 1464469..1464855 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L7ECG3
• Locus tag: VL20_RS07155
• Protein ID: WP_002732589.1
• Coordinates: 1464242..1464469 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VL20_RS31625 (VL20_1733)	1461021..1461158	+	138	WP_284526030.1	hypothetical protein	-
VL20_RS07140 (VL20_1734)	1461334..1462521	+	1188	WP_052276064.1	AAA family ATPase	-
VL20_RS07145 (VL20_1735)	1462546..1463157	+	612	WP_052276065.1	DUF4276 family protein	-
VL20_RS07150 (VL20_1736)	1463721..1464200	+	480	WP_052276066.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
VL20_RS07155 (VL20_1737)	1464242..1464469	+	228	WP_002732589.1	DUF2281 domain-containing protein	Antitoxin
VL20_RS07160 (VL20_1738)	1464469..1464855	+	387	WP_002793526.1	type II toxin-antitoxin system VapC family toxin	Toxin
VL20_RS07165 (VL20_1739)	1464948..1466705	+	1758	WP_052276067.1	arginine--tRNA ligase	-
VL20_RS07170 (VL20_1740)	1467452..1467844	+	393	WP_002767460.1	Mo-dependent nitrogenase C-terminal domain-containing protein	-
VL20_RS07175 (VL20_1741)	1467875..1468633	+	759	WP_052276068.1	hypothetical protein	-
VL20_RS07180 (VL20_1742)	1468638..1468889	+	252	WP_002760725.1	hypothetical protein	-
VL20_RS07185 (VL20_1743)	1468932..1469339	-	408	WP_052276069.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-127)

Antitoxin

• Domain of unknown function DUF2281 (6-74)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14836.14 Da
• Isoelectric Point: 4.8781

>T54038 WP_002793526.1 NZ_CP011339:1464469-1464855 [Microcystis panniformis FACHB-1757]
MTSFLLDTHTFIWLTENDPNLPNNLREEIDFAPEVYVSIVSLWEIAIKLNLGKLALQKSYETIETKLEASDITLLSISFS
DTLKICTLPLYHRDPFDRMLIAQSLNRSLILISKDTKFDAYNVPRKWT

Nucleotide

• Length: 387 bp

>T54038 NZ_CP011339:1464469-1464855 [Microcystis panniformis FACHB-1757]
ATGACATCTTTTCTTCTGGACACCCATACTTTCATCTGGTTAACTGAAAACGATCCTAACTTACCTAATAATCTTCGGGA
GGAAATTGATTTTGCTCCTGAAGTTTATGTCAGTATTGTTAGTCTCTGGGAAATCGCGATTAAGTTAAATTTAGGTAAGT
TAGCCCTACAAAAAAGTTATGAAACCATTGAAACTAAACTAGAAGCATCAGATATAACCTTACTGTCTATATCATTTTCT
GATACTCTAAAAATTTGTACTTTACCCTTGTATCACCGTGATCCATTTGATAGAATGCTAATTGCTCAGTCTCTAAACAG
ATCGCTAATTCTAATTAGTAAAGATACTAAATTTGATGCTTATAATGTACCAAGAAAATGGACATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8801.07 Da
• Isoelectric Point: 4.1064

>AT54038 WP_002732589.1 NZ_CP011339:1464242-1464469 [Microcystis panniformis FACHB-1757]
MNIDTKILETVEAMPELLKIELLHYAEYLMANYQSDVIIEKPSLRKRRSGILQGTFTLPLSDDFDESLEDFQEYM

Nucleotide

• Length: 228 bp

>AT54038 NZ_CP011339:1464242-1464469 [Microcystis panniformis FACHB-1757]
ATGAATATAGACACAAAAATCTTAGAAACTGTCGAAGCAATGCCAGAGTTATTAAAAATAGAGCTGCTTCACTATGCTGA
ATATTTGATGGCCAATTATCAATCAGATGTTATTATAGAGAAACCTTCTCTTAGAAAACGTCGTTCAGGAATTTTACAAG
GTACATTTACCTTACCTTTGTCCGATGATTTTGATGAGTCTTTGGAAGATTTTCAGGAATATATGTGA

Structures

Toxin

• AlphaFold DB: I4HQS4

Antitoxin

• AlphaFold DB: L7ECG3