Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 69936..70461 Replicon plasmid unnamed
Accession NZ_CP011332
Organism Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag AAF13_RS27150 Protein ID WP_001159872.1
Coordinates 69936..70241 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag AAF13_RS27155 Protein ID WP_000813638.1
Coordinates 70243..70461 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF13_RS27125 66108..66524 - 417 WP_001278815.1 plasmid partitioning/stability family protein -
AAF13_RS27130 66517..67497 - 981 WP_000688504.1 plasmid segregation protein ParM -
AAF13_RS27135 67911..68219 - 309 WP_000030204.1 molecular chaperone GroEL -
AAF13_RS27140 68306..68950 - 645 WP_001144032.1 ParA family protein -
AAF13_RS27145 69129..69935 - 807 WP_000016961.1 site-specific integrase -
AAF13_RS27150 69936..70241 - 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
AAF13_RS27155 70243..70461 - 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AAF13_RS27160 71056..71286 + 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AAF13_RS27165 71310..71711 + 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
AAF13_RS27170 71794..71943 - 150 Protein_87 transposase domain-containing protein -
AAF13_RS27185 73035..74012 + 978 WP_001387465.1 RepB family plasmid replication initiator protein -
AAF13_RS27190 74291..75030 - 740 Protein_89 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggA / aggB / aggC / aggD 1..75079 75079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T54031 WP_001159872.1 NZ_CP011332:c70241-69936 [Escherichia coli O104:H4 str. C227-11]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T54031 NZ_CP011332:c70241-69936 [Escherichia coli O104:H4 str. C227-11]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT54031 WP_000813638.1 NZ_CP011332:c70461-70243 [Escherichia coli O104:H4 str. C227-11]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT54031 NZ_CP011332:c70461-70243 [Escherichia coli O104:H4 str. C227-11]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References