Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 436..995 Replicon plasmid unnamed
Accession NZ_CP011332
Organism Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)


Gene name relE Uniprot ID G4VUF8
Locus tag AAF13_RS26730 Protein ID WP_000421273.1
Coordinates 436..711 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag AAF13_RS26735 Protein ID WP_001387467.1
Coordinates 711..995 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF13_RS26725 99..430 - 332 Protein_0 type II toxin-antitoxin system ParD family antitoxin -
AAF13_RS26730 436..711 - 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AAF13_RS26735 711..995 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
AAF13_RS26740 1903..2731 - 829 Protein_3 incFII family plasmid replication initiator RepA -
AAF13_RS26750 2724..2798 - 75 WP_014966221.1 RepA leader peptide Tap -
AAF13_RS32405 2795..2929 - 135 Protein_5 protein CopA/IncA -
AAF13_RS26755 3036..3290 - 255 WP_000083850.1 replication regulatory protein RepA -
AAF13_RS26760 3572..4553 + 982 Protein_7 IS5-like element ISKpn26 family transposase -
AAF13_RS26765 5004..5216 - 213 Protein_8 ANR family transcriptional regulator -
AAF13_RS26770 5281..5958 + 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggA / aggB / aggC / aggD 1..75079 75079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10765.45 Da        Isoelectric Point: 9.9324

>T54030 WP_000421273.1 NZ_CP011332:c711-436 [Escherichia coli O104:H4 str. C227-11]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T54030 NZ_CP011332:c711-436 [Escherichia coli O104:H4 str. C227-11]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT54030 WP_001387467.1 NZ_CP011332:c995-711 [Escherichia coli O104:H4 str. C227-11]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT54030 NZ_CP011332:c995-711 [Escherichia coli O104:H4 str. C227-11]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6H1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References