Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4102099..4102596 Replicon chromosome
Accession NZ_CP011331
Organism Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AAF13_RS20765 Protein ID WP_001173343.1
Coordinates 4102099..4102272 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AAF13_RS20770 Protein ID WP_000398619.1
Coordinates 4102300..4102596 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF13_RS20755 4098769..4100226 + 1458 WP_000856837.1 dipeptide/tripeptide permease DtpC -
AAF13_RS20760 4100463..4101980 + 1518 WP_001295074.1 lysine--tRNA ligase -
AAF13_RS20765 4102099..4102272 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AAF13_RS20770 4102300..4102596 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AAF13_RS20780 4102823..4103096 - 274 Protein_3933 N-acetyltransferase -
AAF13_RS20785 4103108..4103338 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
AAF13_RS20790 4103519..4105150 + 1632 WP_001216486.1 sensor histidine kinase -
AAF13_RS20795 4105147..4105866 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T54026 WP_001173343.1 NZ_CP011331:c4102272-4102099 [Escherichia coli O104:H4 str. C227-11]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T54026 NZ_CP011331:c4102272-4102099 [Escherichia coli O104:H4 str. C227-11]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT54026 WP_000398619.1 NZ_CP011331:c4102596-4102300 [Escherichia coli O104:H4 str. C227-11]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT54026 NZ_CP011331:c4102596-4102300 [Escherichia coli O104:H4 str. C227-11]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References