Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4090066..4090867 | Replicon | chromosome |
| Accession | NZ_CP011331 | ||
| Organism | Escherichia coli O104:H4 str. C227-11 isolate 368 shch | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D3GUT5 |
| Locus tag | AAF13_RS20715 | Protein ID | WP_001094430.1 |
| Coordinates | 4090490..4090867 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D3GUT4 |
| Locus tag | AAF13_RS20710 | Protein ID | WP_001285620.1 |
| Coordinates | 4090066..4090443 (+) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AAF13_RS20680 | 4085269..4086777 | - | 1509 | WP_001189118.1 | group II intron reverse transcriptase/maturase | - |
| AAF13_RS31470 | 4087450..4088189 | - | 740 | Protein_3916 | IS3 family transposase | - |
| AAF13_RS32370 | 4088246..4088371 | + | 126 | Protein_3917 | DUF945 domain-containing protein | - |
| AAF13_RS20695 | 4088713..4089186 | + | 474 | WP_000855057.1 | antirestriction protein | - |
| AAF13_RS20700 | 4089202..4089678 | + | 477 | WP_001387238.1 | RadC family protein | - |
| AAF13_RS20705 | 4089765..4089986 | + | 222 | WP_000692350.1 | DUF987 domain-containing protein | - |
| AAF13_RS20710 | 4090066..4090443 | + | 378 | WP_001285620.1 | type IV toxin-antitoxin system toxin CbtA | Antitoxin |
| AAF13_RS20715 | 4090490..4090867 | + | 378 | WP_001094430.1 | TA system toxin CbtA family protein | Toxin |
| AAF13_RS20720 | 4090864..4091352 | + | 489 | WP_000761715.1 | hypothetical protein | - |
| AAF13_RS20725 | 4091372..4091569 | + | 198 | WP_000772029.1 | DUF957 domain-containing protein | - |
| AAF13_RS20730 | 4091654..4092496 | + | 843 | WP_001290187.1 | DUF4942 domain-containing protein | - |
| AAF13_RS20740 | 4093245..4094783 | + | 1539 | WP_001187182.1 | lysine decarboxylation/transport transcriptional activator CadC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13971.82 Da Isoelectric Point: 6.8608
>T54025 WP_001094430.1 NZ_CP011331:4090490-4090867 [Escherichia coli O104:H4 str. C227-11]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ
Download Length: 378 bp
>T54025 NZ_CP011331:4090490-4090867 [Escherichia coli O104:H4 str. C227-11]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13698.52 Da Isoelectric Point: 5.9505
>AT54025 WP_001285620.1 NZ_CP011331:4090066-4090443 [Escherichia coli O104:H4 str. C227-11]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
Download Length: 378 bp
>AT54025 NZ_CP011331:4090066-4090443 [Escherichia coli O104:H4 str. C227-11]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6M7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 2INW | |
| AlphaFold DB | A0A0E0Y522 |