Detailed information of TA system TA054022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3276913..3277750
• Replicon: chromosome
• Accession: NZ_CP011331
• Organism: Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AAF13_RS16690
• Protein ID: WP_000227784.1
• Coordinates: 3277208..3277750 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AAF13_RS16685
• Protein ID: WP_001297137.1
• Coordinates: 3276913..3277224 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAF13_RS16660	3271933..3272880	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AAF13_RS16665	3272902..3274893	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AAF13_RS16670	3274883..3275497	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AAF13_RS16675	3275497..3275826	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AAF13_RS16680	3275838..3276728	+	891	WP_000971336.1	heme o synthase	-
AAF13_RS16685	3276913..3277224	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AAF13_RS16690	3277208..3277750	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AAF13_RS16695	3277806..3278741	-	936	WP_024174603.1	sel1 repeat family protein	-
AAF13_RS16700	3279149..3280513	+	1365	WP_001000974.1	MFS transporter	-
AAF13_RS16705	3280641..3281132	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AAF13_RS16710	3281300..3282211	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T54022 WP_000227784.1 NZ_CP011331:3277208-3277750 [Escherichia coli O104:H4 str. C227-11]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T54022 NZ_CP011331:3277208-3277750 [Escherichia coli O104:H4 str. C227-11]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT54022 WP_001297137.1 NZ_CP011331:3276913-3277224 [Escherichia coli O104:H4 str. C227-11]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT54022 NZ_CP011331:3276913-3277224 [Escherichia coli O104:H4 str. C227-11]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3