Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3242836..3243454 Replicon chromosome
Accession NZ_CP011331
Organism Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AAF13_RS16520 Protein ID WP_001291435.1
Coordinates 3243236..3243454 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AAF13_RS16515 Protein ID WP_000344800.1
Coordinates 3242836..3243210 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF13_RS16505 3237925..3239118 + 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AAF13_RS16510 3239141..3242290 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AAF13_RS16515 3242836..3243210 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AAF13_RS16520 3243236..3243454 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AAF13_RS16525 3243625..3244176 + 552 WP_000102564.1 maltose O-acetyltransferase -
AAF13_RS16530 3244292..3244762 + 471 WP_000136192.1 YlaC family protein -
AAF13_RS16535 3244926..3246476 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AAF13_RS16540 3246518..3246871 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AAF13_RS16545 3247250..3247561 + 312 WP_000409911.1 MGMT family protein -
AAF13_RS16550 3247592..3248164 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T54021 WP_001291435.1 NZ_CP011331:3243236-3243454 [Escherichia coli O104:H4 str. C227-11]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T54021 NZ_CP011331:3243236-3243454 [Escherichia coli O104:H4 str. C227-11]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT54021 WP_000344800.1 NZ_CP011331:3242836-3243210 [Escherichia coli O104:H4 str. C227-11]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT54021 NZ_CP011331:3242836-3243210 [Escherichia coli O104:H4 str. C227-11]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References