Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3026509..3027163 Replicon chromosome
Accession NZ_CP011322
Organism Escherichia coli strain SQ110

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag SG46_RS14680 Protein ID WP_000244777.1
Coordinates 3026509..3026916 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag SG46_RS14685 Protein ID WP_000354046.1
Coordinates 3026897..3027163 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SG46_RS14660 3022466..3024199 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
SG46_RS14665 3024205..3024915 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
SG46_RS14670 3024940..3025836 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
SG46_RS14675 3025948..3026469 + 522 WP_001055874.1 flavodoxin FldB -
SG46_RS14680 3026509..3026916 - 408 WP_000244777.1 toxin CptA Toxin
SG46_RS14685 3026897..3027163 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
SG46_RS14690 3027406..3028386 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
SG46_RS14695 3028582..3029241 - 660 WP_000250274.1 hemolysin III family protein -
SG46_RS14700 3029405..3029716 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
SG46_RS14705 3029761..3031194 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
SG46_RS14710 3031251..3031994 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T53944 WP_000244777.1 NZ_CP011322:c3026916-3026509 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T53944 NZ_CP011322:c3026916-3026509 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT53944 WP_000354046.1 NZ_CP011322:c3027163-3026897 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT53944 NZ_CP011322:c3027163-3026897 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References