Detailed information of TA system TA053821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2792366..2793408
• Replicon: chromosome
• Accession: NZ_CP011317
• Organism: Pseudomonas aeruginosa strain Carb01 63

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: YQ19_RS13475
• Protein ID: WP_003153636.1
• Coordinates: 2792833..2793408 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: YQ19_RS13470
• Protein ID: WP_003050245.1
• Coordinates: 2792366..2792836 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YQ19_RS13435	2787758..2789176	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
YQ19_RS13440	2789166..2790077	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
YQ19_RS13445	2790074..2790766	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
YQ19_RS13450	2790763..2791161	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
YQ19_RS13455	2791173..2791532	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
YQ19_RS13460	2791549..2791782	-	234	WP_023101201.1	TIGR03758 family integrating conjugative element protein	-
YQ19_RS13465	2791779..2792162	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
YQ19_RS13470	2792366..2792836	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
YQ19_RS13475	2792833..2793408	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
YQ19_RS13480	2793426..2794340	+	915	WP_016852809.1	AAA family ATPase	-
YQ19_RS13485	2794337..2794807	+	471	WP_003090160.1	hypothetical protein	-
YQ19_RS13490	2794804..2795294	+	491	Protein_2725	HORMA domain containing protein	-
YQ19_RS13495	2795294..2796196	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
YQ19_RS13500	2796235..2796960	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2715429..2838890	123461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T53821 WP_003153636.1 NZ_CP011317:2792833-2793408 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T53821 NZ_CP011317:2792833-2793408 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT53821 WP_003050245.1 NZ_CP011317:2792366-2792836 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT53821 NZ_CP011317:2792366-2792836 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68