Detailed information of TA system TA053812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 3966966..3967363
• Replicon: chromosome
• Accession: NZ_CP011304
• Organism: Microcystis aeruginosa NIES-2549

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A6H9FK19
• Locus tag: myaer_RS19470
• Protein ID: WP_071846506.1
• Coordinates: 3966966..3967163 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: myaer_RS19475
• Protein ID: WP_046663291.1
• Coordinates: 3967160..3967363 (-)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer_RS19450	3963211..3964122	+	912	WP_046663289.1	heme A synthase	-
myaer_RS19455	3964274..3965224	+	951	WP_008202059.1	heme o synthase	-
myaer_RS19460	3965317..3966651	-	1335	WP_046663290.1	chromosomal replication initiator protein DnaA	-
myaer_RS19465	3966736..3966969	+	234	Protein_3688	peptide deformylase	-
myaer_RS19470	3966966..3967163	-	198	WP_071846506.1	DUF5615 family PIN-like protein	Toxin
myaer_RS19475	3967160..3967363	-	204	WP_046663291.1	DUF433 domain-containing protein	Antitoxin
myaer_RS19485	3967699..3967923	+	225	WP_071846507.1	hypothetical protein	-
myaer_RS19490	3968038..3968643	-	606	WP_045360674.1	DedA family protein	-
myaer_RS19495	3968735..3970012	-	1278	WP_002732258.1	adenosylhomocysteinase	-
myaer_RS19500	3970157..3970828	-	672	WP_046663297.1	riboflavin synthase	-
myaer_RS19505	3970844..3972139	-	1296	WP_046663298.1	glutamate-5-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-63)

Antitoxin

• Protein of unknown function DUF433 (1-56)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7240.27 Da
• Isoelectric Point: 4.3138

>T53812 WP_071846506.1 NZ_CP011304:c3967163-3966966 [Microcystis aeruginosa NIES-2549]
MTIWVDAHLSPAIATRISTKLEIEAVALRDLGPRDAEDTEIFQVAKAQRAIVMTKDSDFVDLVEQ

Nucleotide

• Length: 198 bp

>T53812 NZ_CP011304:c3967163-3966966 [Microcystis aeruginosa NIES-2549]
ATGACAATTTGGGTTGACGCACATTTGTCTCCTGCAATTGCAACCCGGATTAGCACTAAACTTGAGATTGAAGCTGTGGC
CTTGCGTGATCTTGGACCCAGAGATGCTGAAGATACTGAAATCTTTCAGGTTGCAAAAGCTCAAAGAGCTATCGTAATGA
CCAAAGACAGCGATTTTGTAGATTTAGTTGAGCAGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7073.26 Da
• Isoelectric Point: 5.7055

>AT53812 WP_046663291.1 NZ_CP011304:c3967363-3967160 [Microcystis aeruginosa NIES-2549]
ITVNPRQCGGRACIRGMRILGSDVLALFAAGLSAGEILDEIPDLEADDLKAALLYASRKLNHPVLVA

Nucleotide

• Length: 204 bp

>AT53812 NZ_CP011304:c3967363-3967160 [Microcystis aeruginosa NIES-2549]
ATTACAGTTAATCCTCGGCAGTGCGGTGGTCGTGCCTGTATTCGAGGAATGAGAATCCTGGGATCAGATGTGTTAGCTCT
ATTCGCGGCAGGTTTAAGTGCGGGAGAAATTCTAGATGAAATACCTGATCTTGAGGCAGATGATTTGAAAGCAGCGCTTT
TATATGCCTCACGGAAGCTTAATCACCCAGTTTTAGTGGCATGA

Structures

Toxin

• AlphaFold DB: A0A6H9FK19