Detailed information of TA system TA053811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3469854..3470425
• Replicon: chromosome
• Accession: NZ_CP011304
• Organism: Microcystis aeruginosa NIES-2549

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: myaer_RS17110
• Protein ID: WP_046662963.1
• Coordinates: 3470093..3470425 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I4FQ39
• Locus tag: myaer_RS17105
• Protein ID: WP_002760402.1
• Coordinates: 3469854..3470096 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer_RS21395	3465210..3465353	+	144	WP_158524809.1	hypothetical protein	-
myaer_RS17075	3465363..3465824	-	462	WP_002800691.1	hypothetical protein	-
myaer_RS21400	3465916..3466071	+	156	WP_158524810.1	hypothetical protein	-
myaer_RS17080	3466126..3466521	-	396	Protein_3243	helix-turn-helix domain-containing protein	-
myaer_RS17085	3466536..3467230	+	695	WP_103672892.1	IS1 family transposase	-
myaer_RS17090	3467254..3467859	-	606	Protein_3245	AraC family transcriptional regulator	-
myaer_RS17095	3468290..3468952	+	663	WP_002780534.1	potassium channel family protein	-
myaer_RS17100	3468986..3469762	+	777	WP_046662962.1	DUF2092 domain-containing protein	-
myaer_RS17105	3469854..3470096	+	243	WP_002760402.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
myaer_RS17110	3470093..3470425	+	333	WP_046662963.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
myaer_RS17120	3470784..3471677	+	894	WP_046662964.1	hypothetical protein	-
myaer_RS17125	3471674..3474736	-	3063	WP_046662965.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12596.67 Da
• Isoelectric Point: 10.1581

>T53811 WP_046662963.1 NZ_CP011304:3470093-3470425 [Microcystis aeruginosa NIES-2549]
VTYIPERGDFVRLSFDPQIGHEQMGNRPALVVSPIDFNRKIGFAFVCPVSNTQRQNPFYVKIPDGEAVTGVIMVDQLRSL
DFRARKASFIGKCPERLLQDVFRRIKPILF

Nucleotide

• Length: 333 bp

>T53811 NZ_CP011304:3470093-3470425 [Microcystis aeruginosa NIES-2549]
GTGACATACATTCCAGAACGTGGAGATTTCGTGCGTTTGAGCTTTGACCCACAAATTGGGCATGAGCAAATGGGTAATCG
TCCAGCACTGGTGGTAAGCCCTATTGATTTTAATCGCAAGATTGGTTTTGCCTTTGTCTGCCCGGTGAGCAATACGCAAC
GCCAGAACCCTTTCTACGTCAAGATTCCAGATGGAGAGGCGGTGACAGGAGTTATCATGGTAGATCAACTGCGATCGTTG
GACTTCCGAGCGAGAAAAGCGAGCTTTATCGGCAAGTGTCCAGAACGGTTGCTCCAGGATGTTTTCAGAAGAATTAAGCC
GATTCTGTTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8956.30 Da
• Isoelectric Point: 4.0460

>AT53811 WP_002760402.1 NZ_CP011304:3469854-3470096 [Microcystis aeruginosa NIES-2549]
MKSQIGRWGNSLAIRIPKYAVEALNLNPKDVVECSVENGKLVIELIQALPELSLEELLAEVVESPEPEVDWGRPMGEEVW

Nucleotide

• Length: 243 bp

>AT53811 NZ_CP011304:3469854-3470096 [Microcystis aeruginosa NIES-2549]
ATGAAGTCGCAGATTGGACGCTGGGGTAATTCTTTAGCGATTCGGATTCCTAAGTATGCGGTGGAGGCGCTGAACCTCAA
TCCTAAGGACGTTGTTGAATGTTCTGTGGAAAACGGAAAGCTAGTAATTGAGCTAATTCAAGCCTTGCCTGAGTTAAGCC
TAGAAGAACTACTGGCTGAGGTTGTTGAATCACCGGAGCCGGAAGTGGATTGGGGACGGCCAATGGGAGAGGAGGTTTGG
TGA

Structures

Antitoxin

• AlphaFold DB: I4FQ39