Detailed information of TA system TA053810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3351010..3351791
• Replicon: chromosome
• Accession: NZ_CP011304
• Organism: Microcystis aeruginosa NIES-2549

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A0F6U717
• Locus tag: myaer_RS16465
• Protein ID: WP_046662887.1
• Coordinates: 3351297..3351791 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: I4I6J8
• Locus tag: myaer_RS16460
• Protein ID: WP_002799472.1
• Coordinates: 3351010..3351300 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer_RS16435	3346840..3347154	-	315	WP_046662882.1	hypothetical protein	-
myaer_RS16445	3347526..3348614	-	1089	WP_046662884.1	amidohydrolase	-
myaer_RS16450	3349078..3349656	+	579	WP_046662885.1	hypothetical protein	-
myaer_RS16455	3349794..3350696	+	903	WP_046662886.1	DUF1350 family protein	-
myaer_RS16460	3351010..3351300	+	291	WP_002799472.1	DUF1778 domain-containing protein	Antitoxin
myaer_RS16465	3351297..3351791	+	495	WP_046662887.1	GNAT family N-acetyltransferase	Toxin
myaer_RS16470	3351943..3352173	-	231	WP_046662888.1	hypothetical protein	-
myaer_RS16475	3352170..3352544	-	375	WP_002757633.1	nucleotidyltransferase domain-containing protein	-
myaer_RS16480	3353247..3353399	+	153	WP_080949777.1	DUF4160 domain-containing protein	-
myaer_RS16485	3353501..3353845	+	345	WP_004163940.1	type II toxin-antitoxin system RelE/ParE family toxin	-
myaer_RS16490	3353848..3354147	+	300	WP_012266232.1	helix-turn-helix domain-containing protein	-
myaer_RS16495	3354182..3356479	-	2298	WP_046662889.1	TOMM precursor leader peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17704.36 Da
• Isoelectric Point: 8.1174

>T53810 WP_046662887.1 NZ_CP011304:3351297-3351791 [Microcystis aeruginosa NIES-2549]
MNLTPPEPLASYHSITDFSCGITSLDDWLKRRAYANQASGATRTFVTCVDNRVVGYYAFASGAISIQSANGKFRRNMPNP
IPVVILARLAVDTSYQGQGLGCGLFRDGALRVVKAADTIGSRGIIVHAISEEAKNFYLALGFDVSPLEPMTLMITLNDLR
ACIA

Nucleotide

• Length: 495 bp

>T53810 NZ_CP011304:3351297-3351791 [Microcystis aeruginosa NIES-2549]
ATGAATTTGACACCACCTGAACCTCTGGCTAGTTATCACTCAATCACTGATTTTTCCTGCGGAATTACTTCTCTTGATGA
TTGGTTGAAGCGTCGAGCTTATGCTAATCAAGCCAGTGGTGCAACTAGAACTTTTGTTACCTGCGTTGACAATAGGGTAG
TTGGGTATTACGCCTTTGCTTCCGGGGCGATTAGTATACAATCAGCTAATGGTAAATTTCGGCGGAATATGCCTAACCCA
ATTCCAGTGGTAATTTTGGCACGATTGGCAGTAGATACTTCTTACCAAGGTCAAGGTTTAGGATGCGGTTTATTTCGTGA
TGGTGCGCTACGGGTTGTTAAAGCGGCTGATACCATTGGTAGTCGGGGAATTATTGTTCATGCTATTTCAGAAGAAGCTA
AGAATTTTTATTTGGCTTTAGGTTTTGATGTGTCTCCTCTTGAACCGATGACGTTGATGATTACTCTTAATGATTTACGC
GCTTGTATTGCTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10968.61 Da
• Isoelectric Point: 9.7643

>AT53810 WP_002799472.1 NZ_CP011304:3351010-3351300 [Microcystis aeruginosa NIES-2549]
MSSKTKSKTPRNTLNLRIKPEERNLIDMAAKVQGKNRTDFILEAARNAAEETLLERTIFLASPEAYAEFIALLDAPPQPN
ERLRKTMQTLAPWEKE

Nucleotide

• Length: 291 bp

>AT53810 NZ_CP011304:3351010-3351300 [Microcystis aeruginosa NIES-2549]
ATGAGTAGTAAAACAAAAAGTAAGACTCCGCGTAATACCCTAAATTTGCGAATTAAGCCAGAAGAACGCAATTTGATCGA
TATGGCAGCCAAAGTGCAAGGTAAGAATAGAACTGATTTTATTTTGGAAGCCGCGCGGAATGCTGCGGAGGAAACTTTGC
TCGAACGTACTATTTTTTTGGCTAGTCCAGAGGCTTATGCAGAATTTATCGCACTTCTAGATGCACCACCTCAACCTAAT
GAACGTTTACGCAAAACCATGCAGACGCTCGCACCTTGGGAGAAGGAATGA

Structures

Toxin

• AlphaFold DB: A0A0F6U717

Antitoxin

• AlphaFold DB: I4I6J8