Detailed information of TA system TA053807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2659443..2659939
• Replicon: chromosome
• Accession: NZ_CP011304
• Organism: Microcystis aeruginosa NIES-2549

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I4GVQ6
• Locus tag: myaer_RS13080
• Protein ID: WP_002784337.1
• Coordinates: 2659751..2659939 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: I4IHG6
• Locus tag: myaer_RS13075
• Protein ID: WP_002763050.1
• Coordinates: 2659443..2659739 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer_RS13035	2655527..2656021	-	495	WP_046662409.1	tetratricopeptide repeat protein	-
myaer_RS13040	2656200..2656757	+	558	WP_002763043.1	Uma2 family endonuclease	-
myaer_RS21345	2656778..2656951	+	174	WP_155120481.1	hypothetical protein	-
myaer_RS13045	2657213..2657449	+	237	WP_041803658.1	DUF4160 domain-containing protein	-
myaer_RS13050	2657433..2657681	+	249	WP_002785172.1	DUF2442 domain-containing protein	-
myaer_RS13055	2657865..2658203	-	339	WP_046662410.1	DUF86 domain-containing protein	-
myaer_RS13060	2658200..2658493	-	294	WP_002763046.1	nucleotidyltransferase family protein	-
myaer_RS13065	2658580..2658831	-	252	WP_103672713.1	hypothetical protein	-
myaer_RS13070	2658908..2659252	-	345	WP_002763053.1	hypothetical protein	-
myaer_RS13075	2659443..2659739	-	297	WP_002763050.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
myaer_RS13080	2659751..2659939	-	189	WP_002784337.1	type II toxin-antitoxin system HicA family toxin	Toxin
myaer_RS13085	2659957..2663136	-	3180	WP_046662412.1	serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-91)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7145.39 Da
• Isoelectric Point: 10.8174

>T53807 WP_002784337.1 NZ_CP011304:c2659939-2659751 [Microcystis aeruginosa NIES-2549]
MESREIIKKPKDEGWYQVGTTGSHHHFKHPHKKGKVTVPHPKKDIPLKTLISIEKQAGIKLR

Nucleotide

• Length: 189 bp

>T53807 NZ_CP011304:c2659939-2659751 [Microcystis aeruginosa NIES-2549]
ATGGAAAGTCGAGAAATTATCAAAAAACCGAAGGATGAGGGATGGTATCAAGTAGGTACAACAGGAAGTCATCATCATTT
TAAACATCCCCATAAAAAAGGGAAAGTGACTGTTCCTCACCCTAAAAAGGATATTCCCCTTAAAACCCTAATCAGTATCG
AAAAACAAGCTGGTATTAAATTACGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10797.16 Da
• Isoelectric Point: 4.1825

>AT53807 WP_002763050.1 NZ_CP011304:c2659739-2659443 [Microcystis aeruginosa NIES-2549]
MITYLATVHKDNHSDYGVQFYDFPGCISAGETIEEAKKMATEALKGHISFMLADGDEIPTPSTLETILTDADHQDAIAFL
LIEVSEAILNHQELCVKN

Nucleotide

• Length: 297 bp

>AT53807 NZ_CP011304:c2659739-2659443 [Microcystis aeruginosa NIES-2549]
ATGATTACTTATTTAGCCACCGTTCATAAAGATAACCATAGTGATTATGGGGTACAATTTTATGACTTTCCGGGTTGTAT
TAGTGCAGGAGAAACCATCGAAGAAGCGAAAAAAATGGCAACTGAAGCCTTAAAAGGTCATATTTCCTTCATGTTGGCAG
ATGGGGACGAAATTCCCACCCCTAGCACCCTCGAAACCATTTTAACCGACGCGGATCATCAAGATGCGATCGCTTTTCTG
CTCATTGAGGTATCAGAAGCTATTTTAAACCATCAGGAATTGTGTGTAAAAAACTAG

Structures

Toxin

• AlphaFold DB: I4GVQ6

Antitoxin

• AlphaFold DB: I4IHG6