Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Fic-hicB/Fic-HicB
Location 51875..53022 Replicon plasmid p2
Accession NZ_CP011283
Organism Enterococcus faecium strain E39

Toxin (Protein)


Gene name Fic Uniprot ID R2PRH5
Locus tag XM37_RS14590 Protein ID WP_002300095.1
Coordinates 52417..53022 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7G7IUL1
Locus tag XM37_RS14585 Protein ID WP_002359626.1
Coordinates 51875..52306 (+) Length 144 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XM37_RS14540 46904..47389 + 486 WP_002351368.1 single-stranded DNA-binding protein -
XM37_RS14545 47593..48888 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
XM37_RS14550 49040..49255 - 216 WP_042959779.1 hypothetical protein -
XM37_RS14555 49330..49590 + 261 WP_002296240.1 hypothetical protein -
XM37_RS14560 50031..50237 + 207 WP_002296239.1 hypothetical protein -
XM37_RS14565 50237..50488 + 252 WP_002299286.1 hypothetical protein -
XM37_RS14570 50504..50941 + 438 WP_002326886.1 hypothetical protein -
XM37_RS14580 51386..51565 + 180 WP_002295613.1 hypothetical protein -
XM37_RS16300 51658..51841 + 184 Protein_54 type II toxin-antitoxin system HicA family toxin -
XM37_RS14585 51875..52306 + 432 WP_002359626.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
XM37_RS14590 52417..53022 + 606 WP_002300095.1 Fic family protein Toxin
XM37_RS14595 53038..53613 + 576 WP_002300096.1 recombinase family protein -
XM37_RS14600 53773..53976 + 204 WP_002300097.1 hypothetical protein -
XM37_RS14605 54085..55410 + 1326 WP_002300099.1 Y-family DNA polymerase -
XM37_RS14610 55403..55753 + 351 WP_002300100.1 hypothetical protein -
XM37_RS14615 55750..55929 + 180 WP_002300101.1 hypothetical protein -
XM37_RS14620 56065..56358 + 294 WP_010726525.1 hypothetical protein -
XM37_RS14625 56451..56759 - 309 WP_010726526.1 hypothetical protein -
XM37_RS14630 56772..57728 - 957 WP_010726527.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(47-130)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(10-104)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 23453.73 Da        Isoelectric Point: 4.5712

>T53755 WP_002300095.1 NZ_CP011283:52417-53022 [Enterococcus faecium]
MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTFKGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPV
MYLEQSLNHIDQMPQTSFEEIIKKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL

Download         Length: 606 bp

>T53755 NZ_CP011283:52417-53022 [Enterococcus faecium]
ATGATTTTAGAAAATAAATTGGGTTTAACGAATCAGGTTGAGTTGGCCAAGGCTGAAGAAAAACTCAGTAAGCAAAAAGC
AAAAGAATTATATGATTCTGGAAAAATAAATGAAATCGAAGTCGGCACGTTTAAAGGCTTGTCTGAAATTCATGATTTTC
TCTTTTCAGAAATTTATGATTTTGCAGGAAAAATTCGGACAGTTAATATTGCAAAAGGAAATTTTCGTTTTGCGCCGGTC
ATGTACCTAGAACAATCTTTAAATCATATTGATCAGATGCCGCAAACTTCTTTCGAAGAGATTATTAAGAAATACGTGGA
AATGAATATCGCTCACCCTTTTCGCGAAGGAAACGGGCGCAGTACTAGAATTTGGCTGGATCTCATTTTAAAGGAAGAAT
TGCAAAAAGTGGTCGATTGGAACTTGATCGATAAAGCAGACTATCTTTCTGCAATGGAGCGCAGTCCAATAAATGACTTG
GAAATACGCTATCTCATTTCCAATGCCCTGACTGATAAAATTGACGATCGTGAGTTATATATGAAAGGAATCGATGTCAG
CTACTTCTATGAAGGATACTCTGAGTATACGATAGATGATTTGTAA

Antitoxin


Download         Length: 144 a.a.        Molecular weight: 15809.95 Da        Isoelectric Point: 4.1719

>AT53755 WP_002359626.1 NZ_CP011283:51875-52306 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEELLPEPTPINKIAVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Download         Length: 432 bp

>AT53755 NZ_CP011283:51875-52306 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTTTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGAGCTACTTCCAGAACCAACACCAATCAATAAGATCGCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGTTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R2PRH5

Antitoxin

Source ID Structure
AlphaFold DB A0A7G7IUL1

References