Detailed information of TA system TA053754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 527366..527937
• Replicon: chromosome
• Accession: NZ_CP011281
• Organism: Enterococcus faecium strain E39

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3Y2B8
• Locus tag: XM37_RS02480
• Protein ID: WP_002286801.1
• Coordinates: 527596..527937 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: XM37_RS02475
• Protein ID: WP_033659738.1
• Coordinates: 527366..527596 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XM37_RS02445	522499..523830	+	1332	WP_002286818.1	FAD-containing oxidoreductase	-
XM37_RS02450	523852..524478	+	627	WP_002286816.1	cysteine hydrolase	-
XM37_RS02455	524661..525242	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
XM37_RS02460	525974..526321	+	348	WP_002286808.1	SOS response-associated peptidase family protein	-
XM37_RS02465	526297..526536	+	240	WP_002321938.1	SOS response-associated peptidase family protein	-
XM37_RS02470	526741..527079	-	339	WP_002286804.1	hypothetical protein	-
XM37_RS02475	527366..527596	+	231	WP_033659738.1	hypothetical protein	Antitoxin
XM37_RS02480	527596..527937	+	342	WP_002286801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XM37_RS02485	528970..530157	-	1188	WP_002296840.1	IS256-like element IS16 family transposase	-
XM37_RS02490	530951..531145	+	195	WP_002297028.1	hypothetical protein	-
XM37_RS15475	531303..531585	+	283	Protein_493	transposase	-
XM37_RS02500	531829..532659	-	831	WP_002286789.1	manganese catalase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	528970..530157	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13302.68 Da
• Isoelectric Point: 9.9044

>T53754 WP_002286801.1 NZ_CP011281:527596-527937 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T53754 NZ_CP011281:527596-527937 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8451.32 Da
• Isoelectric Point: 3.9490

>AT53754 WP_033659738.1 NZ_CP011281:527366-527596 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIGDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT53754 NZ_CP011281:527366-527596 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGGAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A829FD66