Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1208309..1209226 Replicon chromosome
Accession NZ_CP011278
Organism Bacillus velezensis strain L-S60

Toxin (Protein)


Gene name spoIISA Uniprot ID -
Locus tag XM40_RS06195 Protein ID WP_025851786.1
Coordinates 1208480..1209226 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag XM40_RS06190 Protein ID WP_003154807.1
Coordinates 1208309..1208479 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XM40_RS06155 1203540..1205162 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
XM40_RS06160 1205175..1205546 + 372 WP_014304856.1 hypothetical protein -
XM40_RS06165 1205552..1205749 + 198 WP_003154819.1 XkdX family protein -
XM40_RS06170 1205806..1206567 + 762 WP_043867020.1 hypothetical protein -
XM40_RS06175 1206619..1206882 + 264 WP_003154815.1 hemolysin XhlA family protein -
XM40_RS06180 1206896..1207159 + 264 WP_003154813.1 phage holin -
XM40_RS06185 1207173..1208051 + 879 WP_025851788.1 N-acetylmuramoyl-L-alanine amidase -
XM40_RS06190 1208309..1208479 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
XM40_RS06195 1208480..1209226 - 747 WP_025851786.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
XM40_RS06200 1209331..1210329 - 999 WP_003154805.1 inorganic phosphate transporter -
XM40_RS06205 1210342..1210959 - 618 WP_003154804.1 DUF47 domain-containing protein -
XM40_RS06210 1211245..1212561 - 1317 WP_003154801.1 amino acid permease -
XM40_RS06215 1212884..1213834 + 951 WP_043867021.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1161649..1266487 104838


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29048.53 Da        Isoelectric Point: 4.7003

>T53751 WP_025851786.1 NZ_CP011278:c1209226-1208480 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T53751 NZ_CP011278:c1209226-1208480 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCGCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGAGTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT53751 WP_003154807.1 NZ_CP011278:c1208479-1208309 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT53751 NZ_CP011278:c1208479-1208309 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References