Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 456941..457578 Replicon chromosome
Accession NZ_CP011278
Organism Bacillus velezensis strain L-S60

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag XM40_RS02345 Protein ID WP_003156187.1
Coordinates 457228..457578 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag XM40_RS02340 Protein ID WP_003156188.1
Coordinates 456941..457222 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XM40_RS02320 453306..453905 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
XM40_RS02325 453998..454363 + 366 WP_014304402.1 holo-ACP synthase -
XM40_RS02330 454528..455535 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
XM40_RS02335 455652..456821 + 1170 WP_003156189.1 alanine racemase -
XM40_RS02340 456941..457222 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
XM40_RS02345 457228..457578 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
XM40_RS02350 457696..458517 + 822 WP_003156182.1 STAS domain-containing protein -
XM40_RS02355 458522..458887 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
XM40_RS02360 458890..459291 + 402 WP_003156178.1 anti-sigma regulatory factor -
XM40_RS02365 459303..460310 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
XM40_RS02370 460374..460703 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
XM40_RS02375 460700..461182 + 483 WP_003156173.1 anti-sigma B factor RsbW -
XM40_RS02380 461148..461936 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
XM40_RS02385 461936..462538 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T53750 WP_003156187.1 NZ_CP011278:457228-457578 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T53750 NZ_CP011278:457228-457578 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT53750 WP_003156188.1 NZ_CP011278:456941-457222 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT53750 NZ_CP011278:456941-457222 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References