Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5363027..5363656 Replicon chromosome
Accession NZ_CP011254
Organism Serratia fonticola strain DSM 4576

Toxin (Protein)


Gene name hicA Uniprot ID A0A0F7HGI5
Locus tag WN53_RS23780 Protein ID WP_021805694.1
Coordinates 5363027..5363215 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0F7D356
Locus tag WN53_RS23785 Protein ID WP_024485643.1
Coordinates 5363243..5363656 (+) Length 138 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WN53_RS23770 5361064..5361693 - 630 WP_024485641.1 TetR/AcrR family transcriptional regulator -
WN53_RS23775 5361902..5362837 + 936 WP_024485642.1 nitronate monooxygenase -
WN53_RS23780 5363027..5363215 + 189 WP_021805694.1 type II toxin-antitoxin system HicA family toxin Toxin
WN53_RS23785 5363243..5363656 + 414 WP_024485643.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
WN53_RS23790 5363689..5364441 - 753 WP_021805696.1 L-cystine ABC transporter ATP-binding protein YecC -
WN53_RS23795 5364445..5365107 - 663 WP_021180972.1 cystine ABC transporter permease -
WN53_RS23800 5365107..5365907 - 801 WP_024485644.1 cystine ABC transporter substrate-binding protein -
WN53_RS23805 5366026..5367012 - 987 WP_046808332.1 D-cysteine desulfhydrase -
WN53_RS23810 5367207..5367716 - 510 WP_024486813.1 flagella biosynthesis regulatory protein FliZ -
WN53_RS23815 5367777..5368499 - 723 WP_021180968.1 RNA polymerase sigma factor FliA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fliZ / fliA 5350023..5368499 18476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7005.17 Da        Isoelectric Point: 11.1589

>T53734 WP_021805694.1 NZ_CP011254:5363027-5363215 [Serratia fonticola]
VKSSELIKRLESHGWVLQRIKGSHHQFKHPNSSLVITVPHPNKDVKLGTLRQIMKDAGLLNS

Download         Length: 189 bp

>T53734 NZ_CP011254:5363027-5363215 [Serratia fonticola]
GTGAAAAGTTCAGAACTGATTAAACGACTCGAAAGCCATGGTTGGGTTTTACAACGGATCAAAGGCAGCCACCATCAATT
CAAACACCCTAACTCATCGTTGGTGATCACCGTGCCTCATCCCAATAAGGATGTGAAATTGGGAACGCTGCGGCAAATCA
TGAAGGATGCAGGGCTGCTTAATTCCTAG

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 15031.77 Da        Isoelectric Point: 4.8403

>AT53734 WP_024485643.1 NZ_CP011254:5363243-5363656 [Serratia fonticola]
MFYPAYIHAEPDGSASGFFPDVPGCYFAGDSLDQTFQDAKSALDAHFELLSEENQAIPRPQTVSFHLAQGNDALNGGQWL
LVDINMDKFDGRAERINITLPHRLLNRIDALVQQHPGYGSRSAFLAAAARNELLKAG

Download         Length: 414 bp

>AT53734 NZ_CP011254:5363243-5363656 [Serratia fonticola]
ATGTTTTATCCCGCTTATATACATGCCGAGCCGGATGGCAGCGCCAGCGGCTTCTTTCCCGATGTGCCAGGCTGCTACTT
TGCCGGAGACAGTCTCGATCAAACCTTCCAGGATGCCAAAAGCGCTCTGGATGCCCACTTTGAATTATTGAGCGAAGAGA
ATCAGGCCATTCCACGCCCACAAACAGTGTCCTTCCATCTGGCTCAGGGCAACGACGCCCTCAACGGCGGGCAGTGGCTG
CTGGTGGATATCAATATGGACAAGTTCGATGGCCGGGCAGAACGCATCAATATCACGTTGCCCCACAGGCTGCTCAACCG
CATTGATGCGCTGGTGCAGCAGCATCCGGGCTATGGCAGCCGCAGCGCATTTCTCGCCGCCGCAGCGCGTAATGAGTTGT
TAAAGGCAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F7HGI5

Antitoxin

Source ID Structure
AlphaFold DB A0A0F7D356

References