Detailed information of TA system TA053724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3254610..3255232
• Replicon: chromosome
• Accession: NZ_CP011254
• Organism: Serratia fonticola strain DSM 4576

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: U2M374
• Locus tag: WN53_RS14410
• Protein ID: WP_021180142.1
• Coordinates: 3254610..3254813 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: U2NAN4
• Locus tag: WN53_RS14415
• Protein ID: WP_021180141.1
• Coordinates: 3254864..3255232 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WN53_RS14385	3250418..3250906	+	489	WP_024484390.1	YbaY family lipoprotein	-
WN53_RS14390	3250960..3251274	-	315	WP_024484391.1	MGMT family protein	-
WN53_RS14400	3251678..3253465	-	1788	WP_024484392.1	adenine deaminase	-
WN53_RS14405	3253602..3254528	+	927	WP_024484393.1	LysR family transcriptional regulator	-
WN53_RS14410	3254610..3254813	-	204	WP_021180142.1	hemolysin expression modulator Hha	Toxin
WN53_RS14415	3254864..3255232	-	369	WP_021180141.1	Hha toxicity modulator TomB	Antitoxin
WN53_RS14420	3255390..3255743	-	354	WP_024484394.1	hypothetical protein	-
WN53_RS14425	3256123..3256815	+	693	WP_024484395.1	ABC transporter ATP-binding protein	-
WN53_RS14430	3256815..3257672	+	858	WP_024484396.1	metal ABC transporter permease	-
WN53_RS14435	3257698..3258576	+	879	WP_024484397.1	zinc ABC transporter substrate-binding protein	-
WN53_RS27330	3258685..3258825	-	141	WP_071784952.1	type B 50S ribosomal protein L36	-
WN53_RS14440	3258838..3259095	-	258	WP_024484398.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8099.48 Da
• Isoelectric Point: 6.9770

>T53724 WP_021180142.1 NZ_CP011254:c3254813-3254610 [Serratia fonticola]
MTKIDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 204 bp

>T53724 NZ_CP011254:c3254813-3254610 [Serratia fonticola]
ATGACAAAAATTGACTATCTGATGCGTTTGCGTAAATGCACCACGATTGAAACCCTTGAGCGAGTGATCGAGAAAAATAA
GTACGAACTCTCTGACGATGAGCTAGAGCTGTTCTACTCTGCCGCCGATCATCGCCTGGCCGAACTGACCATGAACAAGC
TGTACGATAAAATTCCGGTCGCCGTTTGGAAATTTGTAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14097.78 Da
• Isoelectric Point: 4.5140

>AT53724 WP_021180141.1 NZ_CP011254:c3255232-3254864 [Serratia fonticola]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYMDEGDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARTFRLFSGEGVCTTMKT

Nucleotide

• Length: 369 bp

>AT53724 NZ_CP011254:c3255232-3254864 [Serratia fonticola]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAACTCAAATTCTTATGTGAAAATTTGTACGACGAAGGCATCGC
TACCTTGGGTGACAGCCACCATGGCTGGGTTAACGATCCAACGTCCGCCGTCAATCTGCAGCTTAATGAGCTGATCGAAC
ATATTGCTTCGTTTGTAATGAGTTTTAAAATTAAATACATGGACGAAGGTGATTTATCGGAACTGGTCGAAGAGTACCTC
GACGATACCTATACCCTGTTTAGCAGCTACGGTATTAATGACTCCGATTTGCGTCGCTGGCAAAAAACCAAGGCACGCAC
ATTCAGATTGTTCTCAGGAGAGGGCGTCTGTACCACGATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: U2M374

Antitoxin

• AlphaFold DB: U2NAN4