Detailed information of TA system TA053716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 245154..245817
• Replicon: chromosome
• Accession: NZ_CP011254
• Organism: Serratia fonticola strain DSM 4576

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A0F7H6E4
• Locus tag: WN53_RS00955
• Protein ID: WP_024484272.1
• Coordinates: 245154..245498 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: WN53_RS00960
• Protein ID: WP_024484273.1
• Coordinates: 245500..245817 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WN53_RS00935	241318..241824	-	507	WP_024484269.1	tRNA adenosine(34) deaminase TadA	-
WN53_RS00940	242163..243314	+	1152	WP_024484270.1	L-threonine dehydrogenase	-
WN53_RS00945	243354..244001	-	648	WP_024484271.1	phosphatidylglycerophosphatase C	-
WN53_RS00950	244244..245053	+	810	WP_037411923.1	MurR/RpiR family transcriptional regulator	-
WN53_RS00955	245154..245498	+	345	WP_024484272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WN53_RS00960	245500..245817	+	318	WP_024484273.1	helix-turn-helix domain-containing protein	Antitoxin
WN53_RS00965	245896..246156	+	261	WP_024484274.1	YfhL family 4Fe-4S dicluster ferredoxin	-
WN53_RS00970	246409..247368	+	960	WP_046807987.1	IS110 family transposase	-
WN53_RS00975	247799..248179	-	381	WP_024486233.1	holo-ACP synthase	-
WN53_RS00980	248179..248910	-	732	WP_024486234.1	pyridoxine 5'-phosphate synthase	-
WN53_RS00985	248985..249716	-	732	WP_021178899.1	DNA repair protein RecO	-
WN53_RS00990	249725..250633	-	909	WP_037413081.1	GTPase Era	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12489.24 Da
• Isoelectric Point: 9.6410

>T53716 WP_024484272.1 NZ_CP011254:245154-245498 [Serratia fonticola]
MKPLYWVGSSKKDLQSLPEEVQDVFGYALHLAQAGGKHSQTKPLRGFGGAGVLEVVEDFLTDTYRAVYTVKFGDAVYVLH
AFQKKSSAGIATPKPDIDKIRERLKAAENHARGA

Nucleotide

• Length: 345 bp

>T53716 NZ_CP011254:245154-245498 [Serratia fonticola]
ATGAAGCCACTATATTGGGTTGGTAGCAGCAAGAAAGACCTCCAGTCTTTGCCCGAAGAGGTTCAGGATGTTTTCGGCTA
TGCTCTACATCTGGCGCAGGCAGGTGGTAAGCATTCCCAGACGAAACCATTAAGAGGGTTTGGTGGGGCCGGAGTACTAG
AGGTAGTAGAAGACTTTCTTACTGATACTTATCGTGCAGTCTATACCGTTAAGTTTGGCGATGCCGTGTATGTTCTGCAC
GCATTCCAGAAAAAGTCTTCAGCCGGGATCGCCACACCGAAACCGGATATAGACAAAATCCGTGAACGACTGAAAGCCGC
AGAAAATCATGCCAGAGGGGCATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11403.22 Da
• Isoelectric Point: 10.2057

>AT53716 WP_024484273.1 NZ_CP011254:245500-245817 [Serratia fonticola]
MSQDIEVSSGNVYADIGSDGAEEMQVKAQLASTIGDIIKSRRLTQEQAAKLLGMTQPKLSNLLRGQFRGISEAKMLECLT
RLGRDVQIVVGKPRRTPGSLKVVFA

Nucleotide

• Length: 318 bp

>AT53716 NZ_CP011254:245500-245817 [Serratia fonticola]
ATGAGCCAAGATATCGAAGTCAGCAGCGGCAACGTTTATGCCGATATTGGCAGCGACGGCGCTGAAGAAATGCAGGTTAA
AGCCCAACTTGCCAGCACAATTGGCGACATCATCAAAAGTCGCCGCCTGACACAAGAGCAGGCCGCAAAGCTGCTGGGGA
TGACACAGCCCAAGCTGTCTAATCTGTTGCGTGGCCAGTTCCGGGGCATCAGCGAAGCCAAAATGCTAGAATGCCTCACC
CGCTTAGGGCGTGATGTACAAATTGTGGTAGGCAAGCCACGCCGGACACCGGGCAGTCTTAAAGTGGTGTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0F7H6E4