Detailed information of TA system TA053711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1326157..1327073
• Replicon: chromosome
• Accession: NZ_CP011252
• Organism: Bacillus amyloliquefaciens strain MT45

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: WV34_RS07070
• Protein ID: WP_088612661.1
• Coordinates: 1326327..1327073 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: WV34_RS07065
• Protein ID: WP_003154807.1
• Coordinates: 1326157..1326327 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WV34_RS07035	1323067..1323399	+	333	WP_088612657.1	XkdW family protein	-
WV34_RS07040	1323399..1323563	+	165	WP_014470494.1	XkdX family protein	-
WV34_RS07045	1323618..1324415	+	798	WP_088612658.1	phage portal protein	-
WV34_RS07050	1324469..1324732	+	264	WP_088612659.1	hemolysin XhlA family protein	-
WV34_RS07055	1324746..1325009	+	264	WP_013351965.1	phage holin	-
WV34_RS07060	1325023..1325901	+	879	WP_088612660.1	N-acetylmuramoyl-L-alanine amidase	-
WV34_RS07065	1326157..1326327	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
WV34_RS07070	1326327..1327073	-	747	WP_088612661.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
WV34_RS07075	1327182..1328180	-	999	WP_013351968.1	inorganic phosphate transporter	-
WV34_RS07080	1328193..1328810	-	618	WP_003154804.1	DUF47 domain-containing protein	-
WV34_RS07085	1329095..1330411	-	1317	WP_088612662.1	amino acid permease	-
WV34_RS07090	1330733..1331683	+	951	WP_013351970.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1291244..1330411	39167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29084.56 Da
• Isoelectric Point: 4.6839

>T53711 WP_088612661.1 NZ_CP011252:c1327073-1326327 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFTAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T53711 NZ_CP011252:c1327073-1326327 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTATCAGATCATGGTGTGGACAATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCACTATTCACTGCATGGAGACAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTCAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTTACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT53711 WP_003154807.1 NZ_CP011252:c1326327-1326157 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT53711 NZ_CP011252:c1326327-1326157 [Bacillus amyloliquefaciens]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14