Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 474543..475180 Replicon chromosome
Accession NZ_CP011252
Organism Bacillus amyloliquefaciens strain MT45

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag WV34_RS02440 Protein ID WP_003156187.1
Coordinates 474830..475180 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag WV34_RS02435 Protein ID WP_003156188.1
Coordinates 474543..474824 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WV34_RS02415 470909..471508 - 600 WP_088612192.1 rhomboid family intramembrane serine protease -
WV34_RS02420 471601..471966 + 366 WP_088612193.1 holo-ACP synthase -
WV34_RS19885 472343..472438 + 96 WP_151907780.1 hypothetical protein -
WV34_RS02430 473255..474424 + 1170 WP_065521859.1 alanine racemase -
WV34_RS02435 474543..474824 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
WV34_RS02440 474830..475180 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
WV34_RS02445 475301..476122 + 822 WP_045508380.1 STAS domain-containing protein -
WV34_RS02450 476127..476492 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
WV34_RS02455 476495..476895 + 401 Protein_445 anti-sigma regulatory factor -
WV34_RS02460 476907..477914 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
WV34_RS02465 477978..478307 + 330 WP_014469786.1 anti-sigma factor antagonist -
WV34_RS02470 478304..478786 + 483 WP_003156173.1 anti-sigma B factor RsbW -
WV34_RS02475 478752..479540 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
WV34_RS02480 479540..480142 + 603 WP_045508359.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T53710 WP_003156187.1 NZ_CP011252:474830-475180 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T53710 NZ_CP011252:474830-475180 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT53710 WP_003156188.1 NZ_CP011252:474543-474824 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT53710 NZ_CP011252:474543-474824 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References