Detailed information of TA system TA053709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 18727..19268
• Replicon: plasmid pXfb33
• Accession: NZ_CP011251
• Organism: Xanthomonas citri pv. aurantifolii strain FDC 1561

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: TP45_RS22870
• Protein ID: WP_007968778.1
• Coordinates: 18727..19053 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7W9EDN7
• Locus tag: TP45_RS22875
• Protein ID: WP_033478260.1
• Coordinates: 19050..19268 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TP45_RS22830	13961..14242	+	282	WP_017158383.1	type II toxin-antitoxin system YafQ family toxin	-
TP45_RS22835	14563..14964	-	402	WP_007968789.1	hypothetical protein	-
TP45_RS22840	15123..15476	-	354	WP_042676252.1	hypothetical protein	-
TP45_RS22845	15522..16229	-	708	WP_007968786.1	hypothetical protein	-
TP45_RS22850	16226..16681	-	456	WP_007968784.1	hypothetical protein	-
TP45_RS22855	17070..17516	+	447	WP_007968782.1	hypothetical protein	-
TP45_RS22865	18037..18681	+	645	WP_007968779.1	hypothetical protein	-
TP45_RS22870	18727..19053	-	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TP45_RS22875	19050..19268	-	219	WP_033478260.1	antitoxin MazE family protein	Antitoxin
TP45_RS22880	19405..19821	-	417	WP_042676251.1	hypothetical protein	-
TP45_RS22885	19875..20288	-	414	WP_042676250.1	hypothetical protein	-
TP45_RS22890	20299..20874	-	576	WP_042676249.1	phospholipase D family protein	-
TP45_RS22895	20917..21504	-	588	WP_007966857.1	hypothetical protein	-
TP45_RS22900	21550..22437	-	888	WP_011153909.1	lytic transglycosylase domain-containing protein	-
TP45_RS22905	22448..23470	-	1023	WP_007968768.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33022	33022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T53709 WP_007968778.1 NZ_CP011251:c19053-18727 [Xanthomonas citri pv. aurantifolii]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T53709 NZ_CP011251:c19053-18727 [Xanthomonas citri pv. aurantifolii]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8476.58 Da
• Isoelectric Point: 5.0742

>AT53709 WP_033478260.1 NZ_CP011251:c19268-19050 [Xanthomonas citri pv. aurantifolii]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT53709 NZ_CP011251:c19268-19050 [Xanthomonas citri pv. aurantifolii]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7W9EDN7