Detailed information of TA system TA053707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 9583..10118
• Replicon: plasmid pXfb33
• Accession: NZ_CP011251
• Organism: Xanthomonas citri pv. aurantifolii strain FDC 1561

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3C0B3
• Locus tag: TP45_RS22790
• Protein ID: WP_007974210.1
• Coordinates: 9583..9903 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3C0B2
• Locus tag: TP45_RS22795
• Protein ID: WP_011345582.1
• Coordinates: 9903..10118 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TP45_RS22760	5256..5822	-	567	WP_007966097.1	recombinase family protein	-
TP45_RS23100	6073..6642	-	570	WP_164673453.1	hypothetical protein	-
TP45_RS22770	6568..6870	+	303	Protein_8	hypothetical protein	-
TP45_RS22775	7089..8051	+	963	WP_007969091.1	IS1595 family transposase	-
TP45_RS23340	8442..8827	-	386	Protein_10	hypothetical protein	-
TP45_RS22785	9102..9485	-	384	WP_123768333.1	hypothetical protein	-
TP45_RS22790	9583..9903	-	321	WP_007974210.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TP45_RS22795	9903..10118	-	216	WP_011345582.1	antitoxin MazE family protein	Antitoxin
TP45_RS22800	10366..10692	-	327	WP_007974207.1	hypothetical protein	-
TP45_RS22805	10689..11315	-	627	WP_007974205.1	ParA family protein	-
TP45_RS22810	11425..11673	-	249	WP_042806123.1	hypothetical protein	-
TP45_RS22815	11788..12474	+	687	WP_119866679.1	DNA-binding protein	-
TP45_RS22820	12634..13596	+	963	WP_007968792.1	recombinase family protein	-
TP45_RS22825	13705..13974	+	270	WP_017158384.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
TP45_RS22830	13961..14242	+	282	WP_017158383.1	type II toxin-antitoxin system YafQ family toxin	-
TP45_RS22835	14563..14964	-	402	WP_007968789.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33022	33022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11571.48 Da
• Isoelectric Point: 6.2890

>T53707 WP_007974210.1 NZ_CP011251:c9903-9583 [Xanthomonas citri pv. aurantifolii]
MQRGDLVTVSLQGDYGKPRPALVVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Nucleotide

• Length: 321 bp

>T53707 NZ_CP011251:c9903-9583 [Xanthomonas citri pv. aurantifolii]
ATGCAGCGGGGTGACCTTGTGACGGTTTCATTGCAGGGCGACTATGGCAAACCACGGCCCGCCTTGGTCGTTCAATCCGA
CTTGCTCACCGACCTGGAAAGCGTCGTGCTGTGCCCGGTGACAAGCGACCTGCGCAATGCCGCGTTCCGCGTCACCGTGG
AACCGAACCCGGCCAACGGGCTGCGCGCGCTCTCGCAGGTCATGGTGGACAAGCTCTCAACGCTGCCTCGCACCAAGATC
AGCGAACCGTTTGGCCGCCTCGATGATGAGCGGATGAAAGCGATAGACAGGGCGCTCCTGCTGGTCGTTGGCGTTATCTG
A

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8190.20 Da
• Isoelectric Point: 7.2801

>AT53707 WP_011345582.1 NZ_CP011251:c10118-9903 [Xanthomonas citri pv. aurantifolii]
MSSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFAAQVRKQCQNLKGDPAETEVLRFTEEAATHVEGWE

Nucleotide

• Length: 216 bp

>AT53707 NZ_CP011251:c10118-9903 [Xanthomonas citri pv. aurantifolii]
ATGTCATCAATTCACGAATCAAAGGTAGCCCGGCACCGCGAGCGGATGCGCGCTGCGGGGCTACGGCCTGTCCAGTTTTG
GGTGCCCGACACACGCTCTCCCGAGTTCGCGGCGCAAGTGCGCAAGCAGTGCCAAAACCTGAAGGGCGACCCGGCAGAAA
CCGAGGTTTTGCGCTTCACCGAAGAGGCCGCAACGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: Q3C0B3

Antitoxin

• AlphaFold DB: Q3C0B2