Detailed information of TA system TA053595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 6625..7160
• Replicon: plasmid pXfc32
• Accession: NZ_CP011165
• Organism: Xanthomonas citri pv. aurantifolii strain FDC 1609

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3C0B3
• Locus tag: TP50_RS23765
• Protein ID: WP_007974210.1
• Coordinates: 6840..7160 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3C0B2
• Locus tag: TP50_RS23760
• Protein ID: WP_011345582.1
• Coordinates: 6625..6840 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TP50_RS24565	2051..2368	-	318	WP_011153922.1	hypothetical protein	-
TP50_RS23725	2536..2966	+	431	Protein_4	DUF3757 domain-containing protein	-
TP50_RS23735	3149..4111	-	963	WP_007974124.1	recombinase family protein	-
TP50_RS23740	4270..4956	-	687	WP_007974125.1	DNA-binding protein	-
TP50_RS23745	5070..5318	+	249	WP_042806123.1	hypothetical protein	-
TP50_RS23750	5428..6054	+	627	WP_007974205.1	ParA family protein	-
TP50_RS23755	6051..6377	+	327	WP_007974207.1	hypothetical protein	-
TP50_RS23760	6625..6840	+	216	WP_011345582.1	antitoxin MazE family protein	Antitoxin
TP50_RS23765	6840..7160	+	321	WP_007974210.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TP50_RS23770	7829..8647	-	819	WP_088409655.1	hypothetical protein	-
TP50_RS23775	9225..9617	-	393	WP_088409656.1	hypothetical protein	-
TP50_RS23780	9929..10219	+	291	WP_017158353.1	hypothetical protein	-
TP50_RS23785	10247..10618	+	372	WP_007974938.1	TrbC/VirB2 family protein	-
TP50_RS23790	10615..10914	+	300	WP_007968751.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32021	32021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11571.48 Da
• Isoelectric Point: 6.2890

>T53595 WP_007974210.1 NZ_CP011165:6840-7160 [Xanthomonas citri pv. aurantifolii]
MQRGDLVTVSLQGDYGKPRPALVVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Nucleotide

• Length: 321 bp

>T53595 NZ_CP011165:6840-7160 [Xanthomonas citri pv. aurantifolii]
ATGCAGCGGGGTGACCTTGTGACGGTTTCATTGCAGGGCGACTATGGCAAACCACGGCCCGCCTTGGTCGTTCAATCCGA
CTTGCTCACCGACCTGGAAAGCGTCGTGCTGTGCCCGGTGACAAGCGACCTGCGCAATGCCGCGTTCCGCGTCACCGTGG
AACCGAACCCGGCCAACGGGCTGCGCGCGCTCTCGCAGGTCATGGTGGACAAGCTCTCAACGCTGCCTCGCACCAAGATC
AGCGAACCGTTTGGCCGCCTCGATGATGAGCGGATGAAAGCGATAGACAGGGCGCTCCTGCTGGTCGTTGGCGTTATCTG
A

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8190.20 Da
• Isoelectric Point: 7.2801

>AT53595 WP_011345582.1 NZ_CP011165:6625-6840 [Xanthomonas citri pv. aurantifolii]
MSSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFAAQVRKQCQNLKGDPAETEVLRFTEEAATHVEGWE

Nucleotide

• Length: 216 bp

>AT53595 NZ_CP011165:6625-6840 [Xanthomonas citri pv. aurantifolii]
ATGTCATCAATTCACGAATCAAAGGTAGCCCGGCACCGCGAGCGGATGCGCGCTGCGGGGCTACGGCCTGTCCAGTTTTG
GGTGCCCGACACACGCTCTCCCGAGTTCGCGGCGCAAGTGCGCAAGCAGTGCCAAAACCTGAAGGGCGACCCGGCAGAAA
CCGAGGTTTTGCGCTTCACCGAAGAGGCCGCAACGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: Q3C0B3

Antitoxin

• AlphaFold DB: Q3C0B2