Detailed information of TA system TA053594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 33123..33664
• Replicon: plasmid pXfc37
• Accession: NZ_CP011164
• Organism: Xanthomonas citri pv. aurantifolii strain FDC 1609

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: TP50_RS23670
• Protein ID: WP_173668640.1
• Coordinates: 33123..33449 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: TP50_RS23675
• Protein ID: WP_003491150.1
• Coordinates: 33446..33664 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TP50_RS23655	28298..28486	+	189	WP_017162389.1	hypothetical protein	-
TP50_RS23660	28490..30064	+	1575	WP_011345596.1	type IV secretion system DNA-binding domain-containing protein	-
TP50_RS23665	30078..33053	+	2976	WP_088409647.1	conjugative relaxase	-
TP50_RS23670	33123..33449	-	327	WP_173668640.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TP50_RS23675	33446..33664	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
TP50_RS23680	33861..34142	+	282	WP_017157756.1	DUF1778 domain-containing protein	-
TP50_RS23685	34139..34636	+	498	WP_088409648.1	GNAT family N-acetyltransferase	-
TP50_RS23690	34803..35066	-	264	WP_088409649.1	hypothetical protein	-
TP50_RS23695	35159..35572	-	414	WP_088409650.1	hypothetical protein	-
TP50_RS23700	35583..36158	-	576	WP_088409651.1	phospholipase D family protein	-
TP50_RS23705	36201..36788	-	588	WP_007966857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37089	37089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11690.73 Da
• Isoelectric Point: 9.2550

>T53594 WP_173668640.1 NZ_CP011164:c33449-33123 [Xanthomonas citri pv. aurantifolii]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLIAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T53594 NZ_CP011164:c33449-33123 [Xanthomonas citri pv. aurantifolii]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCATTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT53594 WP_003491150.1 NZ_CP011164:c33664-33446 [Xanthomonas citri pv. aurantifolii]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT53594 NZ_CP011164:c33664-33446 [Xanthomonas citri pv. aurantifolii]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACGCGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7